ZO-1 Rekombinanter Antikörper

ZO-1 Rekombinant Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240150D7

Kat-Nr. : CL647-82870-7

Synonyme

TJP1, 240150D7, Tight junction protein 1, Tight junction protein ZO 1, Tight junction protein ZO-1

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  IF Staining of MCF-7 using CL647-82870-7

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 647 ZO-1 antibody (CL647-82870-7, Clone: 240150D7 ) at dilution of 1:200, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, green).

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  IF Staining of MCF-7 using CL647-82870-7

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 647 ZO-1 antibody (CL647-82870-7, Clone: 240150D7 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-82870-7 bindet in IF/ICC ZO-1 und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZO-1 fusion protein Ag33182
• Vollständiger Name: tight junction protein 1 (zona occludens 1)
• Berechnetes Molekulargewicht: 1748 aa, 195 kDa
• Beobachtetes Molekulargewicht: 230 kDa
• GenBank-Zugangsnummer: BC111712
• Gene symbol: ZO-1
• Gene ID (NCBI): 7082
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signaling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).