ZO-2 Rekombinanter Antikörper

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• KD/KO Validated

ZO-2 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

230474D1

Kat-Nr. : 83111-1-RR

Synonyme

TJP2, ZO2, TJP 2, Tight junction protein ZO-2, Tight junction protein ZO 2

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  WB analysis using 83111-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83111-1-RR (ZO-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 83111-1-RR

  WB result of ZO-2 antibody (83111-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZO-2 transfected HeLa cells.

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  IHC staining of mouse colon using 83111-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83111-1-RR (ZO-2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 83111-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83111-1-RR (ZO-2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83111-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83111-1-RR against Human ZO-2 were performed. The affinity constant is 30.8 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, C6-Zellen, HeLa-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83111-1-RR bindet in WB, IHC, ELISA ZO-2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZO-2 fusion protein Ag13534
• Vollständiger Name: tight junction protein 2 (zona occludens 2)
• Berechnetes Molekulargewicht: 134 kDa
• Beobachtetes Molekulargewicht: ~150 kDa
• GenBank-Zugangsnummer: BC027592
• Gene symbol: TJP2
• Gene ID (NCBI): 9414
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.