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ZO-3 Polyklonaler Antikörper

ZO-3 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22116-1-PBS

Synonyme

Tight junction protein 3, Tight junction protein ZO-3, TJP3, ZO3, Zona occludens protein 3

Filter:
  • Western Blot (WB) analysis of MCF-7 cells using ZO-3 Polyclonal antibody (22116-1-AP)

    WB analysis of MCF-7 using 22116-1-AP (same clone as 22116-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 22116-1-AP (ZO-3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22116-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ZO-3 Polyclonal antibody (22116-1-AP)

    WB analysis using 22116-1-AP (same clone as 22116-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22116-1-AP (ZO-3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22116-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of T-47D cells using ZO-3 Polyclonal antibody (22116-1-AP)

    WB analysis of T-47D using 22116-1-AP (same clone as 22116-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 22116-1-AP (ZO-3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22116-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HT-29 cells using ZO-3 Polyclonal antibody (22116-1-AP)

    IP experiment of HT-29 using 22116-1-AP (same clone as 22116-1-PBS)

    IP result of anti-ZO-3 (IP:22116-1-AP, 4ug; Detection:22116-1-AP 1:1000) with HT-29 cells lysate 1760 ug. This data was developed using the same antibody clone with 22116-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

22116-1-PBS bindet in WB, IP, Indirect ELISA ZO-3 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ZO-3 fusion protein Ag17495
Vollständiger Name tight junction protein 3 (zona occludens 3)
Berechnetes Molekulargewicht 952 aa, 105 kDa
Beobachtetes Molekulargewicht 140 kDa
GenBank-ZugangsnummerBC108906
Gene symbol ZO-3
Gene ID (NCBI) 27134
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.