ZO-1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ZO-1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

hamster, human, Hund, Maus, Ratte und mehr (5)

Anwendung

WB, RIP, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

976

Rezensionen (2)

Kat-Nr. : 21773-1-AP

Synonyme

Tight junction protein 1, Tight junction protein ZO 1, TJP1, ZO 1, ZO1, ZO-1, Zona occludens protein 1, Zonula occludens protein 1

---

Galerie der Validierungsdaten

• 

  WB analysis using 21773-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21773-1-AP (ZO-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21773-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21773-1-AP (ZO-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21773-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21773-1-AP (ZO-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 21773-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21773-1-AP (ZO-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 21773-1-AP

  IP result of anti-ZO-1 (IP:21773-1-AP, 4ug; Detection:21773-1-AP 1:500) with HEK-293 cells lysate 2320 ug.

• 

  IHC staining of mouse kidney using 21773-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21773-1-AP (ZO-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 21773-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21773-1-AP (ZO-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 21773-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-1 antibody (21773-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  IF Staining of MDCK using 21773-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 21773-1-AP (ZO-1 antibody) at dilution of 1:600 and 66187-1-Ig (Cytokeratin 18 mouse mAb) at dilution of 1:400, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 21773-1-AP, and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66187-1-Ig.

• 

  IF Staining of HUVEC using 21773-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using ZO-1 antibody (21773-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  IF Staining of MCF-7 using 21773-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-1 antibody (21773-1-AP) at dilution of 1:2000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, green).

• 

  FC experiment of MCF-7 using 21773-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug ZO-1 antibody (21773-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Caco-2-Zellen, COLO 320-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HT-29-Zellen, HUVEC-Zellen, K-562-Zellen, Maushirngewebe, MCF-7-Zellen, Rattenhirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen, HUVEC-Zellen, MDCK-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:1000-1:4000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 697 publications below
• IF: See 368 publications below
• ELISA: See 1 publications below
• FC: See 3 publications below
• RIP: See 1 publications below

Produktinformation

21773-1-AP bindet in WB, RIP, IP, IHC, IF, FC, ELISA ZO-1 und zeigt Reaktivität mit hamster, human, Hund, Maus, Ratten

• Getestete Reaktivität: hamster, human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Hund, Kaninchen, Maus, Ratte, Rind, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZO-1 fusion protein Ag16454
• Vollständiger Name: tight junction protein 1 (zona occludens 1)
• Berechnetes Molekulargewicht: 1748 aa, 195 kDa
• Beobachtetes Molekulargewicht: 230 kDa
• GenBank-Zugangsnummer: BC111712
• Gene symbol: TJP1
• Gene ID (NCBI): 7082
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

What is the function of ZO-1?

Zona Occludens 1 (ZO-1) is a tight junction (TJ) protein found in complexes at cell-cell contacts. The role of ZO-1 is to recruit other TJ proteins.^1,2 The resulting TJ complexes regulate paracellular flow, contribute to apical-basal polarity, and are part of signaling pathways for proliferation and differentiation.³ This protein is useful for highlighting cell-cell contacts both in cultured cells and in tissue samples, outlining cell membranes and specific structures.

Where is ZO-1 expressed?

The protein is localized to the cytoplasmic membrane in most types of cells, particularly where a barrier function is essential. It is common in endothelial cells,⁴ which line the internal surface of blood and lymph vessels, and in epithelial cells,⁵ which form the outer barrier of organs.

ZO-1 is expressed in many tissues including the intestine, kidney, liver, and skeletal muscle cells.

What proteins does ZO-1 interact with?

The molecular weight of ZO-1 is 220kDa⁶ and it contains multiple distinct protein domains that allow bind to other junctional proteins at the cytoplasmic membrane. The N-terminal of ZO-1 can dimerize with other ZO proteins and bind directly to other TJ proteins including claudins, connexions, and JAMs,^7-10 which allows it to assemble TJ complexes. The C-terminal can interact with actin and cortactin, anchoring the TJ complexes to the cytoskeleton.^8,11 This suggests that ZO-1 forms a link between the outer cell-cell contacts and the inner actin.

1.     McNeil, E., Capaldo, C. T. & Macara, I. G. Zonula occludens-1 function in the assembly of tight junctions in Madin-Darby canine kidney epithelial cells. Mol. Biol. Cell 17, 1922-32 (2006).

2.     Kratzer, I. et al. Complexity and developmental changes in the expression pattern of claudins at the blood-CSF barrier. Histochem. Cell Biol. (2012). doi:10.1007/s00418-012-1001-9

3.     Guillemot, L., Paschoud, S., Pulimeno, P., Foglia, A. & Citi, S. The cytoplasmic plaque of tight junctions: A scaffolding and signalling center. Biochim. Biophys. Acta - Biomembr. 1778, 601-613 (2008).

4.     Tornavaca, O. et al. ZO-1 controls endothelial adherens junctions, cell-cell tension, angiogenesis, and barrier formation. J. Cell Biol. 208, 821-38 (2015).

5.     Umeda, K. et al. Establishment and characterization of cultured epithelial cells lacking expression of ZO-1. J. Biol. Chem. 279, 44785-94 (2004).

6.     Stevenson, B. R. Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia. J. Cell Biol. 103, 755-766 (1986).

7.     Itoh, M. et al. Direct Binding of Three Tight Junction-Associated Maguks, Zo-1, Zo-2, and Zo-3, with the Cooh Termini of Claudins. J. Cell Biol. 147, 1351-1363 (1999).

8.     Fanning, A. S., Jameson, B. J., Jesaitis, L. A. & Anderson, J. M. The Tight Junction Protein ZO-1 Establishes a Link between the Transmembrane Protein Occludin and the Actin Cytoskeleton. J. Biol. Chem. 273, 29745-29753 (1998).

9.     Kausalya, P. J., Reichert, M. & Hunziker, W. Connexin45 directly binds to ZO-1 and localizes to the tight junction region in epithelial MDCK cells. FEBS Lett. 505, 92-96 (2001).

10. Itoh, M. et al. Junctional adhesion molecule (JAM) binds to PAR-3. J. Cell Biol. 154, 491-498 (2001).

11. Itoh, M., Nagafuchi, A., Moroi, S. & Tsukita, S. Involvement of ZO-1 in Cadherin-based Cell Adhesion through Its Direct Binding to α Catenin and Actin Filaments. J. Cell Biol. 138, 181-192 (1997).  

Publikationen

Species	Application	Title
mouse		Am J Respir Crit Care Med; Ezrin, a Membrane Cytoskeleton Cross Linker Protein, as a Marker of Epithelial Damage in Asthma.; Authors - Man Jia
human,mouse	IF	Cell Metab; Gut microbiota modulate distal symmetric polyneuropathy in patients with diabetes; Authors - Junpeng Yang
human	IF	Cell Stem Cell; Human branching cholangiocyte organoids recapitulate functional bile duct formation.; Authors - Floris J M Roos
	IF	Microbiome; Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O1; Authors - Hao Chen
mouse	IF	Adv Sci (Weinh); Plasma-Activated Solutions Mitigates DSS-Induced Colitis via Restoring Redox Homeostasis and Reversing Microbiota Dysbiosis; Authors - Tuanhe Sun
human	WB	J Extracell Vesicles; Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.; Authors - Xiaoyu Yu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Xie (Verified Customer) (10-26-2021)

Efficient Labelling from proteintech ZO1 Antibody

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: CaCo-2 cell line

ZO-1 Antibody Immunofluorescence validation (1:150 dilution) in CaCo-2 cell line (Cat no:21773-1-AP)

FH

Samuel (Verified Customer) (08-06-2021)

Clean and strong band at 220-230 kDa in WB (reducing and denaturing conditions). I used the antibody at a 1:2500 dilution in 5% milk, incubation overnight at 4 degrees Celsius. 30ug loaded per sample.

• Applications: Western Blot
• Primary Antibody Dilution: 1/2500
• Cell Tissue Type: SH-SY5Y, HEPG2, HEK293. 30ug each

ZO-1 Antibody Western Blot validation (1/2500 dilution) in SH-SY5Y, HEPG2, HEK293. 30ug each (Cat no:21773-1-AP)

Verwandte Produkte

Occludin Polyclonal antibody

13409-1-AP

ZO-2 Polyclonal antibody

18900-1-AP

Connexin-43 Polyclonal antibody

15386-1-AP

E-cadherin Polyclonal antibody

20874-1-AP

Cortactin Polyclonal antibody

11381-1-AP