ZO1 Rekombinanter Antikörper

ZO1 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240150A1

Kat-Nr. : 82870-2-PBS

Synonyme

TJP1, ZO-1, Tight junction protein 1, Tight junction protein ZO 1, Tight junction protein ZO-1

---

• 

  Sandwich ELISA standard curve of MP00349-4

  Sandwich ELISA standard curve of MP00349-4, Human ZO-1 Recombinant Matched Antibody Pair - PBS only. 82870-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag33182. 82870-9-PBS was HRP conjugated as the detection antibody. Range: 125-8000 pg/mL.

• 

  Sample test of MP00349-4

  Serum of nine individual healthy human donors was measured. The ZO-1 concentration of detected samples was determined to be 352.4 pg/mL with a range of 83.4 - 1,272.8 pg/mL.

• 

  Sample test of MP00349-4

  The mean ZO-1 concentration was determined to be 7.5 ng/mL in bEnd.3 cell extract based on a 1.8 mg/mL extract load.

• 

  Affinity and Kinetic Characterization of 82870-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 82870-2-PBS against Human ZO1 was performed. The affinity constant is 1.05 nM.

---

Geprüfte Anwendungen

Produktinformation

82870-2-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test ZO1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZO1 fusion protein Ag33182
• Vollständiger Name: tight junction protein 1 (zona occludens 1)
• Berechnetes Molekulargewicht: 1748 aa, 195 kDa
• GenBank-Zugangsnummer: BC111712
• Gene symbol: ZO-1
• Gene ID (NCBI): 7082
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.