ZO-1 Rekombinanter Antikörper

ZO-1 Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

240150D7

Kat-Nr. : 82870-7-RR

Synonyme

TJP1, 240150D7, Tight junction protein 1, Tight junction protein ZO 1, Tight junction protein ZO-1

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  WB analysis using 82870-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82870-7-RR (ZO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82870-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82870-7-RR (ZO-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 82870-7-RR

  IP result of anti-ZO-1 (IP:82870-7-RR, 4ug; Detection:82870-7-RR 1:4000) with HEK-293 cells lysate 1610 ug.

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  IHC staining of mouse colon using 82870-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82870-7-RR (ZO-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 82870-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82870-7-RR (ZO-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 82870-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82870-7-RR (ZO-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 82870-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82870-7-RR (ZO-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 82870-7-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82870-7-RR (ZO-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 82870-7-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82870-7-RR (ZO-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse colon using 82870-7-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using ZO-1 antibody (82870-7-RR, Clone: 240150D7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 82870-7-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZO-1 antibody (82870-7-RR, Clone: 240150D7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  IF Staining of Caco-2 using 82870-7-RR

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using ZO-1 antibody (82870-7-RR, Clone: 240150D7 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  IF Staining of MDCK using 82870-7-RR

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using ZO-1 antibody (82870-7-RR, Clone: 240150D7 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  IF Staining of U2OS using 82870-7-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZO-1 antibody (82870-7-RR, Clone: 240150D7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of MCF-7 using 82870-7-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZO-1 antibody (82870-7-RR, Clone: 240150D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  FC experiment of MCF-7 using 82870-7-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ZO-1 Recombinant antibody (82870-7-RR, Clone:240150D7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82870-7-RR

  Biolayer interferometry (BLl) kinetic assays of 82870-7-RR against Human ZO-1 were performed. The affinity constant is 16.2 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, bEnd.3-Zellen, Caco-2-Zellen, HepG2-Zellen, HT-29-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe, humanes Plazenta-Gewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Kolongewebe
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen, Caco-2-Zellen, MDCK-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82870-7-RR bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA ZO-1 und zeigt Reaktivität mit human, Hund, Maus

• Getestete Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZO-1 fusion protein Ag33182
• Vollständiger Name: tight junction protein 1 (zona occludens 1)
• Berechnetes Molekulargewicht: 1748 aa, 195 kDa
• Beobachtetes Molekulargewicht: 230 kDa
• GenBank-Zugangsnummer: BC111712
• Gene symbol: ZO-1
• Gene ID (NCBI): 7082
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tight junction (or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium (PMID: 20066090). ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells (PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin (PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs (MAGUKs). It is a pivotal tight junction protein and may be involved in signaling mechanisms regulating cell proliferation and differentiation (PMID: 22782886).