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ZP2 Polyklonaler Antikörper

ZP2 Polyklonal Antikörper für WB, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21832-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using ZP2 Polyclonal antibody (21832-1-AP)

    WB analysis using 21832-1-AP (same clone as 21832-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21832-1-AP (ZP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21832-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse ovary tissue using ZP2 Polyclonal antibody (21832-1-AP)

    IF Staining of mouse ovary using 21832-1-AP (same clone as 21832-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using ZP2 antibody (21832-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21832-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

21832-1-PBS bindet in WB, IF-P, Indirect ELISA ZP2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ZP2 fusion protein Ag16386
Vollständiger Name zona pellucida glycoprotein 2 (sperm receptor)
Berechnetes Molekulargewicht 745 aa, 82 kDa
Beobachtetes Molekulargewicht75-82 kDa
GenBank-ZugangsnummerBC096304
Gene symbol ZP2
Gene ID (NCBI) 7783
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZP2 is a major component of the zona pellucida to which sperm bind prior to penetrating the zona matrix and fusing with the egg. Following fertilization and cortical granule exocytosis, ZP2 is cleaved by ovastacin after which sperm do not bind to the zona pellucida. The zona pellucida and ovastacin proteins transverse the endomembrane system during oogenesis during which passage ZP2 remains intact. Secreted ZP2 is incorporated into the extracellular zona pellucida surrounding ovulated eggs and is cleaved in the zona matrix surrounding 2-cell embryos. (PMID: 28114310)