ZRANB2 Polyklonaler Antikörper

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• KD/KO Validated

ZRANB2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 24816-1-AP

Synonyme

Zinc finger protein 265, Zinc finger, splicing, ZIS, ZIS1, ZIS2, ZNF265, ZRANB2

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 24816-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24816-1-AP (ZRANB2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 24816-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24816-1-AP (ZRANB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24816-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24816-1-AP (ZRANB2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

24816-1-AP bindet in WB, IHC, ELISA ZRANB2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZRANB2 fusion protein Ag20487
• Vollständiger Name: zinc finger, RAN-binding domain containing 2
• Berechnetes Molekulargewicht: 330 aa, 37 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC039814
• Gene symbol: ZRANB2
• Gene ID (NCBI): 9406
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZRANB2, also named as ZIS and ZNF265, is a splice factor required for alternative splicing of TRA2B/SFRS10 transcripts. It may interfere with constitutive 5'-splice site selection. The calculated MW of ZRANB2 is 330aa, 37 kDa or 320aa, 36 kDa. We always got the ~48 kDa in our detection. The immunogen of this antibody is 1-241aa. No other protein can be recognized by this antibody from the sequence BLAST results.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; ZRANB2 and SYF2-mediated splicing programs converging on ECT2 are involved in breast cancer cell resistance to doxorubicin.; Authors - Iris Tanaka
human	WB	Aging (Albany NY); LncRNA FAM181A-AS1 promotes gliomagenesis by sponging miR-129-5p and upregulating ZRANB2.; Authors - Xin Jiang
human	WB,IHC	J Exp Clin Cancer Res; ZRANB2/SNHG20/FOXK1 Axis regulates Vasculogenic mimicry formation in glioma.; Authors - Xiaozhi Li ; KD Validated