ZRANB3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ZRANB3 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 23111-1-AP

Synonyme

ZRANB3

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 23111-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23111-1-AP (ZRANB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 23111-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23111-1-AP (ZRANB3 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 23111-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23111-1-AP (ZRANB3 Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HEK-293 using 23111-1-AP

  Immunofluorescent analysis of HEK-293 cells using 23111-1-AP (ZRANB3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 10 publications below
• WB: See 15 publications below

Produktinformation

23111-1-AP bindet in WB, IHC, IF, ELISA ZRANB3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZRANB3 fusion protein Ag19407
• Vollständiger Name: zinc finger, RAN-binding domain containing 3
• Berechnetes Molekulargewicht: 1079 aa, 123 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC064616
• Gene symbol: ZRANB3
• Gene ID (NCBI): 84083
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZRANB3, also named Annealing helicase 2 or DNA annealing helicase and endonuclease ZRANB3, is a 1079 amino acid protein, which contains one RanBP2-type zinc finger, one helicase C-terminal domain, one HNH domain and one helicase ATP-binding domain. ZRANB3 localizes in the nucleus and belongs to the SNF2/RAD54 helicase family. ZRANB3 as a DNA annealing helicase and endonuclease is required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Excessive reactive oxygen species induce transcription-dependent replication stress; Authors - Martin Andrs ; KD Validated
human	WB	Mol Cell; Active DNA damage eviction by HLTF stimulates nucleotide excision repair.; Authors - Marvin van Toorn ; KD Validated
human	WB	Mol Cell; Replication Fork Slowing and Reversal upon DNA Damage Require PCNA Polyubiquitination and ZRANB3 DNA Translocase Activity.; Authors - Marko Vujanovic ; KD Validated
human	WB	Mol Cell; Fork Cleavage-Religation Cycle and Active Transcription Mediate Replication Restart after Fork Stalling at Co-transcriptional R-Loops.; Authors - Nagaraja Chappidi ; KD Validated
human	WB	Mol Cell; CtIP-Mediated Fork Protection Synergizes with BRCA1 to Suppress Genomic Instability upon DNA Replication Stress.; Authors - Sara Przetocka ; KD Validated
human	WB	Nat Commun; Replication fork reversal triggers fork degradation in BRCA2-defective cells.; Authors - Sofija Mijic ; KO Validated