ZW10 Polyklonaler Antikörper

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• KD/KO Validated

ZW10 Polyklonal Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24561-1-PBS

Synonyme

HZW10

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  WB analysis using 24561-1-AP (same clone as 24561-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24561-1-AP (ZW10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24561-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 24561-1-AP (same clone as 24561-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24561-1-AP (ZW10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24561-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 24561-1-AP (same clone as 24561-1-PBS)

  WB result of ZW10 antibody (24561-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZW10 transfected HeLa cells. This data was developed using the same antibody clone with 24561-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 24561-1-AP (same clone as 24561-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 24561-1-AP (ZW10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24561-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 24561-1-AP (same clone as 24561-1-PBS)

  IP result of anti-ZW10 (IP:24561-1-AP, 4ug; Detection:24561-1-AP 1:500) with Jurkat cells lysate 3200ug. This data was developed using the same antibody clone with 24561-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 24561-1-AP (same clone as 24561-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ZW10 antibody (24561-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24561-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 24561-1-AP (same clone as 24561-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ZW10 (24561-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 24561-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24561-1-PBS bindet in WB, IF/ICC, FC (Intra), IP, ELISA ZW10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZW10 fusion protein Ag19138
• Vollständiger Name: ZW10, kinetochore associated, homolog (Drosophila)
• Berechnetes Molekulargewicht: 779 aa, 89 kDa
• Beobachtetes Molekulargewicht: 88-90 kDa
• GenBank-Zugangsnummer: BC128264
• Gene symbol: ZW10
• Gene ID (NCBI): 9183
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZW10 is the human homolog of the Drosophila melanogaster Zw10 protein. ZW10 is localized to kinetochores and kinetochore-associated microtubules and is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis(PMID: 17102640). ZW10 proteins in Drosophila and HeLa cells display a similar and intriguing cell cycle-dependent intracellular distribution. ZW10 protein first becomes localized to the kinetochore at prometaphase, but then appears to move onto the kinetochore microtubules of the spindle at metaphase, and then back to the kinetochore at anaphase(PMID: 9700164).