ZXDC Polyklonaler Antikörper

ZXDC Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 20530-1-AP

Synonyme

Zinc finger protein ZXDC, ZXD family zinc finger C, ZXD like zinc finger protein, ZXDC, ZXDL

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  WB analysis of human kidney using 20530-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 20530-1-AP (ZXDC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20530-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20530-1-AP (ZXDC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20530-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20530-1-AP (ZXDC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 20530-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20530-1-AP (ZXDC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 20530-1-AP

  Immunofluorescent analysis of Hela cells, using ZXDC antibody 20530-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe, A431-Zellen, HEK-293-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

20530-1-AP bindet in WB, IHC, IF/ICC, ELISA ZXDC und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZXDC fusion protein Ag14392
• Vollständiger Name: ZXD family zinc finger C
• Berechnetes Molekulargewicht: 858 aa, 90 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC002940
• Gene symbol: ZXDC
• Gene ID (NCBI): 79364
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Nat Commun; Cell-fate transition and determination analysis of mouse male germ cells throughout development.; Authors - Jiexiang Zhao
human	WB,IHC	iScience; ZXDC enhances cervical cancer metastasis through IGF2BP3-mediated activation of RhoA/ROCK signaling; Authors - Yifang Mao
human,mouse	WB	Cancer Cell; The crosstalk between macrophages and cancer cells potentiates pancreatic cancer cachexia; Authors - Mingyang Liu