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ZYG11A Polyklonaler Antikörper

ZYG11A Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30597-1-PBS

Synonyme

Protein zyg 11 homolog A, ZYG11

Filter:
  • Western Blot (WB) analysis of various lysates using ZYG11A Polyclonal antibody (30597-1-AP)

    WB analysis using 30597-1-AP (same clone as 30597-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30597-1-AP (ZYG11A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30597-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ZYG11A Polyclonal antibody (30597-1-AP)

    WB analysis using 30597-1-AP (same clone as 30597-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30597-1-AP (ZYG11A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30597-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using ZYG11A Polyclonal antibody (30597-1-AP)

    IF Staining of K-562 using 30597-1-AP (same clone as 30597-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ZYG11A antibody (30597-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30597-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30597-1-PBS bindet in WB, IF/ICC, Indirect ELISA ZYG11A und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ZYG11A fusion protein Ag30166
Vollständiger Name zyg-11 homolog A (C. elegans)
Berechnetes Molekulargewicht 86 kDa
Beobachtetes Molekulargewicht48 kDa
GenBank-ZugangsnummerNM_001004339
Gene symbol ZYG11A
Gene ID (NCBI) 440590
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZYG11A is a member of the Zyg11 protein family that functions as a target recruitment subunit of an E3 ubiquitin ligase complex playing a central role in the degradation of a regulatory protein involved in cell cycle regulation. ZYG11A is over-expressed in NSCLC tumor tissues and correlates with more aggressive clinical characteristics. Also ZYG11A gene constitutes a novel target for IGF1 action in endometrial cancer cells (PMID: 26771237, 31320996). This antibody is specific to isoform 2 of ZYG11A with a predicted molecular mass of 48 kDa.