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- KD/KO Validated
Zyxin Polyklonaler Antikörper
Zyxin Polyklonal Antikörper für IF, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (2)
Anwendung
WB, IP, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10330-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | A549-Zellen, HeLa-Zellen, MCF-7-Zellen |
| Erfolgreiche Detektion in IF | A549-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunfluoreszenz (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IF | See 5 publications below |
| IP | See 1 publications below |
Produktinformation
10330-1-AP bindet in WB, IP, IF, ELISA Zyxin und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human, Kaninchen, Maus |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | Zyxin fusion protein Ag0419 |
| Vollständiger Name | zyxin |
| Berechnetes Molekulargewicht | 80 kDa |
| Beobachtetes Molekulargewicht | 78 kDa |
| GenBank-Zugangsnummer | BC008743 |
| Gene symbol | ZYX |
| Gene ID (NCBI) | 7791 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin (ZYX) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for Zyxin antibody 10330-1-AP | Protokoll herunterladen |
| IF protocol for Zyxin antibody 10330-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
J Cell Biol The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration. | ||
Cell Death Dis Essential role of zyxin in platelet biogenesis and glycoprotein Ib-IX surface expression
| ||
Cell Prolif Zyxin-involved actin regulation is essential in the maintenance of vinculin focal adhesion and chondrocyte differentiation status.
| ||
J Biol Chem O-GlcNAc transferase promotes the nuclear localization of the focal adhesion-associated protein Zyxin to regulate UV-induced cell death. | ||
Am J Physiol Gastrointest Liver Physiol Targeted disruption of the Lasp-1 gene is linked to increases in histamine-stimulated gastric HCl secretion. | ||
Physiol Genomics Lasp1 gene disruption is linked to enhanced cell migration and tumor formation. |
