Zyxin Polyklonaler Antikörper

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• KD/KO Validated

Zyxin Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Rezensionen (1)

Kat-Nr. : 10330-1-AP

Synonyme

ZYX, Zyxin-2

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  WB analysis using 10330-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10330-1-AP (Zyxin antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 10330-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10330-1-AP (Zyxin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 10330-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Zyxin antibody (10330-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Produktinformation

10330-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Zyxin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Zyxin fusion protein Ag0419
• Vollständiger Name: zyxin
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC008743
• Gene symbol: Zyxin
• Gene ID (NCBI): 7791
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin (ZYX) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.

Publikationen

Species	Application	Title
mouse	IF	J Cell Biol; The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.; Authors - Natalia Schiefermeier
mouse,human	WB,IF	Cell Death Dis; Essential role of zyxin in platelet biogenesis and glycoprotein Ib-IX surface expression; Authors - Rong Yan ; KD Validated
rabbit	IF	Cell Prolif; Zyxin-involved actin regulation is essential in the maintenance of vinculin focal adhesion and chondrocyte differentiation status.; Authors - Gaoming Li ; KD Validated
human	WB,IP,IF	J Biol Chem; O-GlcNAc transferase promotes the nuclear localization of the focal adhesion-associated protein Zyxin to regulate UV-induced cell death.; Authors - Yuhang Zhao
mouse	WB	Am J Physiol Gastrointest Liver Physiol; Targeted disruption of the Lasp-1 gene is linked to increases in histamine-stimulated gastric HCl secretion.; Authors - Chew Catherine S CS
mouse	WB,IF	Physiol Genomics; Lasp1 gene disruption is linked to enhanced cell migration and tumor formation.; Authors - Zhang Han H

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alex (Verified Customer) (08-20-2025)

Excellent staining of adhesions and actin filament tips. Consistent with literature-reported fluorescent protein constructs. In image: A - Paxillin (from another manufacturer) B - Zyxin (10330-1-AP. 1:200) C - Phalloidin (from another manufacturer) D - Merged (G = Paxillin; R = Zyxin; gray = phalloidin)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: neural

Zyxin Antibody Immunofluorescence validation (1:200 dilution) in neural (Cat no:10330-1-AP)

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