Zyxin Monoklonaler Antikörper

Zyxin Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4D7E7

Kat-Nr. : 60254-1-PBS

Synonyme

4D7E7, ZYX, Zyxin-2

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  WB analysis of HeLa using 60254-1-Ig (same clone as 60254-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60254-1-Ig (Zyxin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60254-1-Ig (same clone as 60254-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60254-1-Ig (Zyxin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 60254-1-Ig (same clone as 60254-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 60254-1-Ig (Zyxin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer slide using 60254-1-Ig (Zyxin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 60254-1-Ig (same clone as 60254-1-PBS)

  Immunofluorescent analysis of HeLa cells using 60254-1-Ig (Zyxin antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 60254-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60254-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Zyxin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Zyxin fusion protein Ag0419
• Vollständiger Name: zyxin
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC008743
• Gene symbol: Zyxin
• Gene ID (NCBI): 7791
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin (ZYX) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.