Alpha Actinin Rekombinanter Antikörper

Alpha Actinin Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250833F4

Kat-Nr. : 86340-2-PBS

Synonyme

ACTN1, Alpha actinin 1, actin, actinin, alpha 1, ACTN 1

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  WB analysis using 86340-2-RR (same clone as 86340-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86340-2-RR (Alpha Actinin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86340-2-PBS in a different storage buffer formulation.

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  Sample test of MP02360-1

  The mean Alpha Actinin concentration was determined to be 254.6 ng/mL in NIH/3T3 cell extract based on a 1.4 mg/mL extract load.

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  IHC staining of mouse heart using 86340-2-RR (same clone as 86340-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86340-2-RR (Alpha Actinin antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86340-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 86340-2-RR (same clone as 86340-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86340-2-RR (Alpha Actinin antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86340-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 86340-2-RR (same clone as 86340-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha Actinin antibody (86340-2-RR, Clone: 250833F4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86340-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 86340-2-RR (same clone as 86340-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha Actinin antibody (86340-2-RR, Clone: 250833F4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86340-2-PBS in a different storage buffer formulation.

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  IF Staining of H9C2 using 86340-2-RR (same clone as 86340-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using Alpha Actinin antibody (86340-2-RR, Clone: 250833F4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86340-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02360-1

  Cytometric bead array standard curve of MP02360-1, Alpha Actinin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86340-2-PBS. Detection antibody: 86340-1-PBS. Standard: Ag1859. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 86340-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86340-2-RR against Human Alpha Actinin were performed. The affinity constant is 1.94 nM.

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Geprüfte Anwendungen

Produktinformation

86340-2-PBS bindet in WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA, Sample test Alpha Actinin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Alpha Actinin fusion protein Ag1859
• Vollständiger Name: actinin, alpha 1
• Berechnetes Molekulargewicht: 103 kDa
• Beobachtetes Molekulargewicht: 100-105 kDa
• GenBank-Zugangsnummer: BC015766
• Gene symbol: Alpha Actinin
• Gene ID (NCBI): 87
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.