Beta Actin Monoklonaler Antikörper

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• KD/KO Validated

Beta Actin Monoklonal Antikörper für WB

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Affe, hamster, human, Maus, Ratte, Zebrafisch

Anwendung

WB, IP

Konjugation

HRP Fluorescent Dye

Publikationen

39

CloneNo.

2D4H5

Kat-Nr. : HRP-66009

Synonyme

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cell and organelle markers, Cytoskeleton Marker, F actin, HRP-66009, PS1TP5BP1, β actin

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Galerie der Validierungsdaten

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  WB analysis using HRP-66009

  Various lysates were subjected to SDS PAGE followed by western blot with HRP-66009 (beta Actin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:50000

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 37 publications below
• IP: See 1 publications below

Produktinformation

HRP-66009 bindet in WB, IP Beta Actin und zeigt Reaktivität mit Affe, hamster, human, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: Affe, hamster, human, Maus, Ratte, Zebrafisch
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: actin, beta
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: NM_001101
• Gene symbol: ACTB
• Gene ID (NCBI): 60
• Konjugation: HRP Fluorescent Dye
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody is conjugated with HRP. It can recognize all the actins. The isotype of this antibody is IgG2b.

Publikationen

Species	Application	Title
	WB	Nat Commun; Cytosine base editors induce off-target mutations and adverse phenotypic effects in transgenic mice; Authors - Nana Yan
mouse	WB	Protein Cell; Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm; Authors - Kuisheng Liu
human	WB	Nat Commun; Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.; Authors - Hu Lei
human,mouse	WB	Nat Commun; SOD1 regulates ribosome biogenesis in KRAS mutant non-small cell lung cancer.; Authors - Xiaowen Wang
human	WB	Bone Res; Restoring the dampened expression of the core clock molecule BMAL1 protects against compression-induced intervertebral disc degeneration.; Authors - Dong Wang
mouse	WB	JCI Insight; CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling; Authors - Marta Bueno