TGFBI/BIGH3 Rekombinanter Antikörper

TGFBI/BIGH3 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243108H4

Kat-Nr. : 80805-3-PBS

Synonyme

TGFBI, TGFBI / BIGH3, Beta ig h3, Beta ig-h3, BIGH3

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  WB analysis of mouse eye using 80805-3-RR (same clone as 80805-3-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 80805-3-RR (beta IG-H3/TGFBI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80805-3-PBS in a different storage buffer formulation.

• 

  WB analysis of rat eye using 80805-3-RR (same clone as 80805-3-PBS)

  rat eye tissue were subjected to SDS PAGE followed by western blot with 80805-3-RR (beta IG-H3/TGFBI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80805-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 80805-3-RR (same clone as 80805-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80805-3-RR (beta IG-H3/TGFBI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80805-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array sample test of MP02279-1

  The mean TGFBI/BIGH3 concentration was determined to be 3586.8 ng/mL in human serum.

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  Cytometric bead array standard curve of MP02279-1

  Cytometric bead array standard curve of MP02279-1, TGFBI/BIGH3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80805-3-PBS. Detection antibody: 80805-2-PBS. Standard: Eg1233. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 80805-3-RR

  Biolayer interferometry (BLl) kinetic assays of 80805-3-RR against Human TGFBI/BIGH3 were performed. The affinity constant is 2.62 nM.

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Geprüfte Anwendungen

Produktinformation

80805-3-PBS bindet in WB, Cytometric bead array, Indirect ELISA TGFBI/BIGH3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TGFBI/BIGH3 fusion protein Eg1233
• Vollständiger Name: transforming growth factor, beta-induced, 68kDa
• Berechnetes Molekulargewicht: 75kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: NM_000358.3
• Gene symbol: TGFBI
• Gene ID (NCBI): 7045
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).