c-Cbl Monoklonaler Antikörper

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c-Cbl Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F6D1

Kat-Nr. : 66576-1-PBS

Synonyme

C CBL, CBL, CBL2, c-Cbl, Proto oncogene c Cbl, RING finger protein 55, RNF55

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  WB analysis using 66576-1-Ig (same clone as 66576-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66576-1-Ig (c-Cbl antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66576-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Daudi using 66576-1-Ig (same clone as 66576-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66576-1-Ig (CBL antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66576-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66576-1-Ig (same clone as 66576-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66576-1-Ig (CBL antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66576-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66576-1-Ig (same clone as 66576-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66576-1-Ig (CBL antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66576-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66576-1-PBS bindet in WB, IHC, Indirect ELISA c-Cbl und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: c-Cbl fusion protein Ag23033
• Vollständiger Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence
• Berechnetes Molekulargewicht: 906 aa, 100 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa
• GenBank-Zugangsnummer: BC132733
• Gene symbol: CBL
• Gene ID (NCBI): 867
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

c-Cbl (Casitas B-lineage lymphoma) is a critical regulator that controls immature or follicular helper T cell development, B cell development, immune tolerance, angiogenesis, or tumorigenesis (PMID: 33962939). c-Cbl also plays a pivotal role in regulating dendritic cell (DC) function via negatively modulating IL-12 production after Toll-like receptor (TLR) stimulation (PMID: 21799517).