c-MYC Rekombinanter Antikörper

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• KD/KO Validated

c-MYC Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

2B17

Kat-Nr. : 80845-1-PBS

Synonyme

MYC, 2B17, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39

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  WB analysis of A549 using 80845-1-RR (same clone as 80845-1-PBS)

  WB result of MYC antibody (80845-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYC transfected A549 cells. This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

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  WB analysis using 80845-1-RR (same clone as 80845-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 80845-1-RR (c-MYC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

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  WB analysis using 80845-1-RR (same clone as 80845-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80845-1-RR (MYC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 80845-1-RR (same clone as 80845-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 80845-1-RR (c-MYC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 80845-1-RR (same clone as 80845-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80845-1-RR (c-MYC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80845-1-RR (same clone as 80845-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MYC antibody (80845-1-RR, Clone: 2B17 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 80845-1-RR (same clone as 80845-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MYC antibody (80845-1-RR, Clone: 2B17 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 80845-1-RR (same clone as 80845-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYC antibody (80845-1-RR, Clone: 2B17 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 80845-1-RR (same clone as 80845-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug c-MYC Recombinant antibody (80845-1-RR, Clone:2B17) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

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  ChIP experiment of HeLa using 80845-1-RR (same clone as 80845-1-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of c-MYC (80845-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80845-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80845-1-RR against Human c-MYC were performed. The affinity constant is 2.03 nM.

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Geprüfte Anwendungen

Produktinformation

80845-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR c-MYC und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: c-MYC fusion protein Ag1263
• Vollständiger Name: v-myc myelocytomatosis viral oncogene homolog (avian)
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 50-65 kDa
• GenBank-Zugangsnummer: BC000141
• Gene symbol: MYC
• Gene ID (NCBI): 4609
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.