cGAS Polyklonaler Antikörper

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cGAS Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29958-1-PBS

Synonyme

m cGAS, Mb21d1, mcGAS, m-cGAS

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  WB analysis using 29958-1-AP (same clone as 29958-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29958-1-AP (cGAS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 29958-1-AP (same clone as 29958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 29958-1-AP (same clone as 29958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 29958-1-AP (same clone as 29958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 29958-1-AP (same clone as 29958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 29958-1-AP (same clone as 29958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 29958-1-AP (same clone as 29958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29958-1-AP (cGAS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

• 

  IF Staining of THP-1 using 29958-1-AP (same clone as 29958-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using cGAS antibody (29958-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 29958-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29958-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA cGAS und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: cGAS fusion protein Ag31677
• Vollständiger Name: RIKEN cDNA E330016A19 gene
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC145651
• Gene symbol: cGAS
• Gene ID (NCBI): 214763
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

cGAS (cyclic GMP-AMP synthase) is a cytosolic DNA sensor that serves to mount an immune response against the invasion of microbial pathogens such as viruses. cGAS normally resides as an inactive protein in the cell. Upon binding to DNA, cGAS undergoes a conformational change to an active state and produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP, which is subsequently detected by the cyclic-dinucleotide sensor STING, an ~40 kDa dimeric transmembrane protein at the endoplasmic reticulum (ER). cGAS not only is found in the cytosol but has a multifaceted cellular distribution that involves localization at the cell membrane and in the nucleus (PMID: 32424334). The calculated molecular weight of cGAS is 58 kDa. With post-translational modification, the MW of cGAS will be migrated to 70 kDa.