cGAS Monoklonaler Antikörper

cGAS Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2G2C5

Kat-Nr. : 68640-1-PBS

Synonyme

2'3'-cGAMP synthase, 2G2C5, C6orf150, cGAMP synthase, EC:2.7.7.86

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  WB analysis using 68640-1-Ig (same clone as 68640-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68640-1-Ig (cGAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68640-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68640-1-Ig (same clone as 68640-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68640-1-Ig (cGAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68640-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68640-1-Ig (same clone as 68640-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68640-1-Ig (cGAS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68640-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 68640-1-Ig (same clone as 68640-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using cGAS antibody (68640-1-Ig, Clone: 2G2C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68640-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68640-1-PBS bindet in WB, IF/ICC, Indirect ELISA cGAS und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: cGAS fusion protein Ag30074
• Vollständiger Name: chromosome 6 open reading frame 150
• Berechnetes Molekulargewicht: 496 aa, 54 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC113608
• Gene symbol: cGAS
• Gene ID (NCBI): 115004
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.