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cGAS Rekombinanter Antikörper

cGAS Rekombinant Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

240964E2

Kat-Nr. : 84045-1-PBS

Synonyme

240964E2, m cGAS, Mb21d1, mcGAS, m-cGAS

Filter:
  • Western Blot (WB) analysis of various lysates using cGAS Recombinant antibody (84045-1-RR)

    WB analysis using 84045-1-RR (same clone as 84045-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84045-1-RR (cGAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84045-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using cGAS Recombinant antibody (84045-1-RR)

    IP experiment of NIH/3T3 using 84045-1-RR (same clone as 84045-1-PBS)

    IP result of anti-cGAS (IP:84045-1-RR, 4ug; Detection:84045-1-RR 1:600) with NIH/3T3 cells lysate 1920 ug. This data was developed using the same antibody clone with 84045-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using cGAS Recombinant antibody (84045-1-RR)

    IF Staining of RAW 264.7 using 84045-1-RR (same clone as 84045-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using cGAS antibody (84045-1-RR, Clone: 240964E2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84045-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

84045-1-PBS bindet in WB, IF/ICC, IP, ELISA cGAS und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen cGAS fusion protein Ag31677
Vollständiger Name RIKEN cDNA E330016A19 gene
Berechnetes Molekulargewicht58 kDa
Beobachtetes Molekulargewicht58 kDa
GenBank-ZugangsnummerBC145651
Gene symbol cGAS
Gene ID (NCBI) 214763
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

cGAS (cyclic GMP-AMP synthase) is a cytosolic DNA sensor that serves to mount an immune response against the invasion of microbial pathogens such as viruses. cGAS normally resides as an inactive protein in the cell. Upon binding to DNA, cGAS undergoes a conformational change to an active state and produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP, which is subsequently detected by the cyclic-dinucleotide sensor STING, an ~40 kDa dimeric transmembrane protein at the endoplasmic reticulum (ER). cGAS not only is found in the cytosol but has a multifaceted cellular distribution that involves localization at the cell membrane and in the nucleus (PMID: 32424334). The calculated molecular weight of cGAS is 58 kDa. With post-translational modification, the MW of cGAS will be migrated to 70 kDa.