cGAS Rekombinanter Antikörper

cGAS Rekombinant Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

240964E2

Kat-Nr. : 84045-1-RR

Synonyme

240964E2, m cGAS, Mb21d1, mcGAS, m-cGAS

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  WB analysis using 84045-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84045-1-RR (cGAS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 84045-1-RR

  IP result of anti-cGAS (IP:84045-1-RR, 4ug; Detection:84045-1-RR 1:600) with NIH/3T3 cells lysate 1920 ug.

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  IF Staining of RAW 264.7 using 84045-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using cGAS antibody (84045-1-RR, Clone: 240964E2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, C2C12-Zellen, RAW 264.7-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

84045-1-RR bindet in WB, IHC, IF/ICC, IP, ELISA cGAS und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: cGAS fusion protein Ag31677
• Vollständiger Name: RIKEN cDNA E330016A19 gene
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC145651
• Gene symbol: cGAS
• Gene ID (NCBI): 214763
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

cGAS (cyclic GMP-AMP synthase) is a cytosolic DNA sensor that serves to mount an immune response against the invasion of microbial pathogens such as viruses. cGAS normally resides as an inactive protein in the cell. Upon binding to DNA, cGAS undergoes a conformational change to an active state and produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP, which is subsequently detected by the cyclic-dinucleotide sensor STING, an ~40 kDa dimeric transmembrane protein at the endoplasmic reticulum (ER). cGAS not only is found in the cytosol but has a multifaceted cellular distribution that involves localization at the cell membrane and in the nucleus (PMID: 32424334). The calculated molecular weight of cGAS is 58 kDa. With post-translational modification, the MW of cGAS will be migrated to 70 kDa.

Publikationen

Species	Application	Title
human,mouse	WB,IHC,IF	Int Immunopharmacol; cGAS-STING mediates neutrophil extracellular traps-induced EMT in myositis-associated interstitial lung disease: STING as a potential therapeutic target; Authors - Yingyue Feng