cIAP1 Monoklonaler Antikörper

cIAP1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

1H3F1

Kat-Nr. : 66626-1-Ig

Synonyme

API1, BIRC2, MIHB, RNF48, 1H3F1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HSC-T6-Zellen, Jurkat-Zellen, Maus-Skelettmuskelgewebe, Hausschwein-Hirngewebe, Rattenhirngewebe
Erfolgreiche Detektion in IHChumanes Milzgewebe, humanes Lungenkarzinomgewebe, humanes Lymphomgewebe, humanes Tonsillitisgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunhistochemie (IHC)IHC : 1:150-1:600
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66626-1-Ig bindet in WB, IHC, IF/ICC, IP, ELISA cIAP1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen cIAP1 fusion protein Ag21398
Vollständiger Name baculoviral IAP repeat-containing 2
Berechnetes Molekulargewicht 618 aa, 70 kDa
Beobachtetes Molekulargewicht 70 kDa
GenBank-ZugangsnummerBC016174
Gene symbol cIAP1
Gene ID (NCBI) 329
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BIRC2 (also known as cIAP1) is a member of the inhibitor of apoptosis protein (IAP) family. The inhibitor of apoptosis (IAP) proteins are a family of anti-apoptotic regulators found in viruses and metazoans. BIRC2 is a nuclear shuttling protein, whose subcellular localization is mediated by the CRM1-dependent nuclear export pathway (PMID: 15265700). The protein is regulated transcriptionally and can be inhibited by mitochondrial proteins released in the cytoplasm upon apoptotic stimuli (PMID: 15187025). BIRC2 is also believed to be a critical regulator of vascular integrity and endothelial cell survival, thereby providing an additional target pathway for the control of angiogenesis and blood vessel homeostasis during embryogenesis, regeneration and tumorigenesis (PMID: 17934460).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for cIAP1 antibody 66626-1-IgProtokoll herunterladen
IHC protocol for cIAP1 antibody 66626-1-IgProtokoll herunterladenl
IF protocol for cIAP1 antibody 66626-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell Death Dis

Cellular FLICE-like inhibitory protein (cFLIP) critically maintains apoptotic resistance in human lens epithelial cells.

Authors - Jingru Huangfu
humanWB

Chem Biol Interact

Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65

Authors - Jingwen Tan
mouse,humanWB,IP,IHC,IF

J Exp Clin Cancer Res

TRIM56 promotes malignant progression of glioblastoma by stabilizing cIAP1 protein

Authors - Xu Yang
mouseWB,IF

JACC Basic Transl Sci

Bag3 Regulates Mitochondrial Function and the Inflammasome Through Canonical and Noncanonical Pathways in the Heart

Authors - JuFang Wang
mouse,humanWB

Mol Cell

Palmitoylation licenses RIPK1 kinase activity and cytotoxicity in the TNF pathway

Authors - Na Zhang
humanWB

Ecotoxicol Environ Saf

Arsenic-induced circASXL1 regulates 16HBE cell proliferation through the P65 signaling axis

Authors - Xuefei Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Shubham (Verified Customer) (10-04-2019)

Good

  • Applications: Immunoprecipitation,
  • Cell Tissue Type: AC16
cIAP1 Antibody Immunoprecipitation, validation ( dilution) in AC16 (Cat no:66626-1-Ig)