cIAP1 Monoklonaler Antikörper

cIAP1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H3F1

Kat-Nr. : 66626-1-PBS

Synonyme

API1, BIRC2, MIHB, RNF48, 1H3F1

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  WB analysis using 66626-1-Ig (same clone as 66626-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66626-1-Ig (cIAP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

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  WB analysis using 66626-1-Ig (same clone as 66626-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66626-1-Ig (cIAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66626-1-Ig (same clone as 66626-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66626-1-Ig (cIAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66626-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA cIAP1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: cIAP1 fusion protein Ag21398
• Vollständiger Name: baculoviral IAP repeat-containing 2
• Berechnetes Molekulargewicht: 618 aa, 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC016174
• Gene symbol: cIAP1
• Gene ID (NCBI): 329
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BIRC2 (also known as cIAP1) is a member of the inhibitor of apoptosis protein (IAP) family. The inhibitor of apoptosis (IAP) proteins are a family of anti-apoptotic regulators found in viruses and metazoans. BIRC2 is a nuclear shuttling protein, whose subcellular localization is mediated by the CRM1-dependent nuclear export pathway (PMID: 15265700). The protein is regulated transcriptionally and can be inhibited by mitochondrial proteins released in the cytoplasm upon apoptotic stimuli (PMID: 15187025). BIRC2 is also believed to be a critical regulator of vascular integrity and endothelial cell survival, thereby providing an additional target pathway for the control of angiogenesis and blood vessel homeostasis during embryogenesis, regeneration and tumorigenesis (PMID: 17934460).