cIAP2 Rekombinanter Antikörper

cIAP2 Rekombinant Antikörper für WB, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242214E11

Kat-Nr. : 84779-4-PBS

Synonyme

BIRC3, 242214E11, Baculoviral IAP repeat-containing protein 3, BIRC 3, C IAP2

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  WB analysis using 84779-4-RR (same clone as 84779-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84779-4-RR (cIAP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84779-4-PBS in a different storage buffer formulation.

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  IP experiment of Raji using 84779-4-RR (same clone as 84779-4-PBS)

  IP result of anti-cIAP2 (IP:84779-4-RR, 4ug; Detection:84779-4-RR 1:1000) with Raji cells lysate 1600 ug. This data was developed using the same antibody clone with 84779-4-PBS in a different storage buffer formulation.

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  FC experiment of Raji using 84779-4-RR (same clone as 84779-4-PBS)

  1x10^6 Raji cells were intracellularly stained with 0.25 ug cIAP2 Recombinant antibody (84779-4-RR, Clone:242214E11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84779-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84779-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84779-4-RR against Human cIAP2 were performed. The affinity constant is 46.8 pM.

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Geprüfte Anwendungen

Produktinformation

84779-4-PBS bindet in WB, FC (Intra), IP, Indirect ELISA cIAP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: cIAP2 fusion protein Ag20500
• Vollständiger Name: baculoviral IAP repeat-containing 3
• Berechnetes Molekulargewicht: 604 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC027485
• Gene symbol: cIAP2
• Gene ID (NCBI): 330
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

cIAP2 also known as BIRC3, is a member of the Inhibitor of Apoptosis Protein (IAP) family. cIAP2 is induced by inflammatory stimuli and plays a critical role in regulating adaptive and innate immune responses, cell death, and the production of inflammatory mediators. Its dysregulation is associated with various diseases, including cancer and neuroinflammatory conditions. Understanding the mechanisms by which cIAP2 functions and interacts with other cellular components is essential for developing targeted therapies.