cIAP2 Rekombinanter Antikörper

cIAP2 Rekombinant Antikörper für WB, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

242214E11

Kat-Nr. : 84779-4-RR

Synonyme

BIRC3, 242214E11, Baculoviral IAP repeat-containing protein 3, BIRC 3, C IAP2

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  WB analysis using 84779-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84779-4-RR (cIAP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 84779-4-RR

  IP result of anti-cIAP2 (IP:84779-4-RR, 4ug; Detection:84779-4-RR 1:1000) with Raji cells lysate 1600 ug.

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  FC experiment of Raji using 84779-4-RR

  1x10^6 Raji cells were intracellularly stained with 0.25 ug cIAP2 Recombinant antibody (84779-4-RR, Clone:242214E11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 84779-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84779-4-RR against Human cIAP2 were performed. The affinity constant is 46.8 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, Raji-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in FC (Intra): Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84779-4-RR bindet in WB, FC (Intra), IP, ELISA cIAP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: cIAP2 fusion protein Ag20500
• Vollständiger Name: baculoviral IAP repeat-containing 3
• Berechnetes Molekulargewicht: 604 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC027485
• Gene symbol: cIAP2
• Gene ID (NCBI): 330
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

cIAP2 also known as BIRC3, is a member of the Inhibitor of Apoptosis Protein (IAP) family. cIAP2 is induced by inflammatory stimuli and plays a critical role in regulating adaptive and innate immune responses, cell death, and the production of inflammatory mediators. Its dysregulation is associated with various diseases, including cancer and neuroinflammatory conditions. Understanding the mechanisms by which cIAP2 functions and interacts with other cellular components is essential for developing targeted therapies.