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calreticulin Rekombinanter Antikörper

calreticulin Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243011A9

Kat-Nr. : 85593-6-PBS

Synonyme

Calr, Calregulin, CRP55, Endoplasmic reticulum resident protein 60, ERp60

Filter:
  • Western Blot (WB) analysis of various lysates using calreticulin Recombinant antibody (85593-6-RR)

    WB analysis using 85593-6-RR (same clone as 85593-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85593-6-RR (Calr antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85593-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using calreticulin Recombinant antibody (85593-6-RR)

    IF Staining of U2OS using 85593-6-RR (same clone as 85593-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using calreticulin antibody (85593-6-RR, Clone: 243011A9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85593-6-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85593-6-PBS bindet in WB, IF/ICC, Indirect ELISA calreticulin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen calreticulin fusion protein Eg3005
Vollständiger Name calreticulin
Berechnetes Molekulargewicht48 kDa
Beobachtetes Molekulargewicht50-55 kDa
GenBank-ZugangsnummerNM_007591.3
Gene symbol Calr
Gene ID (NCBI) 12317
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CALR, also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.