calreticulin Polyklonaler Antikörper

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• KD/KO Validated

calreticulin Polyklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 555 Fluorescent Dye

Publikationen(1)

Kat-Nr. : CL555-27298

Synonyme

CALR, Calregulin, CRT

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  IF Staining of HepG2 using CL555-27298

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 555 calreticulin antibody (CL555-27298) at dilution of 1:200, CL488-phalloidin (green).

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  IF Staining of SKOV-3 using CL555-27298

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite® Plus 555 calreticulin antibody (CL555-27298) at dilution of 1:200.

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  IF Staining of U2OS using CL555-27298

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 555 calreticulin antibody (CL555-27298) at dilution of 1:200.

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  FC experiment of HeLa using CL555-27298

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 555 Anti-Human calreticulin (CL555-27298) (red), or 0.4 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen, HepG2-Zellen, SKOV-3-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL555-27298 bindet in IF/ICC, FC (Intra) calreticulin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: calreticulin fusion protein Ag26064
• Vollständiger Name: calreticulin
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC002500
• Gene symbol: Calreticulin
• Gene ID (NCBI): 811
• Konjugation: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 554 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CALR, also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is an ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 60 kD by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.

Publikationen

Species	Application	Title
mouse	IF	Biomaterials; A cuproptosis-based nanomedicine suppresses triple negative breast cancers by regulating tumor microenvironment and eliminating cancer stem cells; Authors - Chen Xiao