citrate synthase Monoklonaler Antikörper

citrate synthase Monoklonal Antikörper für WB, IHC, IF/ICC, IF-Fro, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-Fro, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F9G6

Kat-Nr. : 67784-1-PBS

Synonyme

CS, 2F9G6, CISY, Citrate, Citrate synthase, mitochondrial

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  WB analysis using 67784-1-Ig (same clone as 67784-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67784-1-Ig (citrate synthase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

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  WB analysis using 67784-1-Ig (same clone as 67784-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67784-1-Ig (CS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67784-1-Ig (same clone as 67784-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67784-1-Ig (citrate synthase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67784-1-Ig (same clone as 67784-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67784-1-Ig (citrate synthase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse breast cancer using 67784-1-Ig (same clone as 67784-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67784-1-Ig (same clone as 67784-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67784-1-Ig (same clone as 67784-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using citrate synthase antibody (67784-1-Ig, Clone: 2F9G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67784-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67784-1-PBS bindet in WB, IHC, IF/ICC, IF-Fro, Indirect ELISA citrate synthase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: citrate synthase fusion protein Ag9255
• Vollständiger Name: citrate synthase
• Berechnetes Molekulargewicht: 466 aa, 52 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC010106
• Gene symbol: Citrate Synthase
• Gene ID (NCBI): 1431
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.