Cleaved Caspase 3 Polyklonaler Antikörper

Cleaved Caspase 3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

32

Kat-Nr. : 25128-1-AP

Synonyme

active capsase 3, Apopain, CASP 3, CASP3, Caspase 3, Caspase3, Cleaved Caspase 3; P17, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

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Galerie der Validierungsdaten

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  WB analysis using 25128-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25128-1-AP (cleaved Caspase 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 25128-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25128-1-AP (Cleaved Caspase 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25128-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25128-1-AP (Cleaved Caspase 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 29 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Produktinformation

25128-1-AP bindet in WB, IHC, IF, ELISA Cleaved Caspase 3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: caspase 3, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 17 kDa, 19 kDa
• GenBank-Zugangsnummer: NM_032991
• Gene symbol: CASP3
• Gene ID (NCBI): 836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize p17, p19 and p32 of Caspase 3. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3, it can recognize the 17 and 19 kDa cleaved-caspase 3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826).

Publikationen

Species	Application	Title
human	WB	Small; A Responsive Nanorobot Modulates Intracellular Zinc Homeostasis to Amplify Mitochondria-Targeted Phototherapy; Authors - Xinyuan Zhou
chicken	WB,IF	Sci Total Environ; MitomiR-1736-3p regulates copper-induced mitochondrial pathway apoptosis by inhibiting AATF in chicken hepatocytes; Authors - Yuanxu Li
human,mouse	WB,IHC	J Transl Med; Cirsiliol induces autophagy and mitochondrial apoptosis through the AKT/FOXO1 axis and influences methotrexate resistance in osteosarcoma; Authors - Mengliang Luo
mouse	WB	Liver Int; PPARβ/δ activation protects against hepatic ischaemia-reperfusion injury; Authors - Baolin Qian
human	WB	Arch Pharm Res; Selumetinib overcomes gefitinib primary and acquired resistance by regulating MIG6/STAT3 in NSCLC; Authors - Xiaoping Song
human	WB	Cell Signal; Phosphorylated Hsp27 promotes adriamycin resistance in breast cancer cells through regulating dual phosphorylation of c-Myc; Authors - Xiaowen Bi