cyclin B1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

cyclin B1 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

1F3G11

Kat-Nr. : 67686-1-PBS

Synonyme

CCNB1, 1F3G11, CCNB, Cyclin B, G2/mitotic specific cyclin B1

---

• 

  WB analysis using 67686-1-Ig (same clone as 67686-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67686-1-Ig (cyclin B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67686-1-Ig (same clone as 67686-1-PBS)

  WB result of cyclin B1 antibody (67686-1-Ig; 1:3200; incubated at room temperature for 1.5 hours) with sh-Control and sh-cyclin B1 transfected HeLa cells. This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67686-1-Ig (same clone as 67686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67686-1-Ig (cyclin B1 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67686-1-Ig (same clone as 67686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67686-1-Ig (cyclin B1 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skin using 67686-1-Ig (same clone as 67686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 67686-1-Ig (cyclin B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67686-1-Ig (same clone as 67686-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using cyclin B1 antibody (67686-1-Ig, Clone: 1F3G11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67686-1-Ig (same clone as 67686-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using cyclin B1 antibody (67686-1-Ig, Clone: 1F3G11 ) at dilution of 1:650 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HT-29 using 67686-1-Ig (same clone as 67686-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using cyclin B1 antibody (67686-1-Ig, Clone: 1F3G11 ) at dilution of 1:650 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 67686-1-Ig (same clone as 67686-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug cyclin B1 Monoclonal antibody (67686-1-Ig, Clone:1F3G11)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67686-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), ELISA cyclin B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: cyclin B1 fusion protein Ag29444
• Vollständiger Name: cyclin B1
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC006510
• Gene symbol: Cyclin B1
• Gene ID (NCBI): 891
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.