domain-I-of-FIZ-1 Polyklonaler Antikörper

domain-I-of-FIZ-1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15826-1-AP

Synonyme

FIZ1, ZNF798, Flt3-interacting zinc finger protein 1, FIZ 1

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  WB analysis of human kidney using 15826-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15826-1-AP (domain-I-of-FIZ-1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 15826-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 15826-1-AP (domain-I-of-FIZ-1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 15826-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15826-1-AP (domain-I-of-FIZ-1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15826-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15826-1-AP (domain-I-of-FIZ-1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 15826-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15826-1-AP (domain-I-of-FIZ-1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 15826-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15826-1-AP (domain-I-of-FIZ-1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15826-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15826-1-AP (domain-I-of-FIZ-1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15826-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15826-1-AP (domain-I-of-FIZ-1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15826-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15826-1-AP (domain-I-of-FIZ-1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 15826-1-AP

  Immunofluorescent analysis of () fixed HepG2 cells using 15826-1-AP (domain-I-of-FIZ-1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15826-1-AP bindet in WB, IHC, IF/ICC, ELISA domain-I-of-FIZ-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: FLT3-interacting zinc finger 1
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: NM_032836
• Gene symbol: FIZ1
• Gene ID (NCBI): 84922
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.