dsRNA Rekombinanter Antikörper

dsRNA Rekombinant Antikörper für IF/ICC, FC (Intra), Dot Blot, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

n/a

Anwendung

IF/ICC, FC (Intra), Dot Blot, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250708A2

Kat-Nr. : 85780-2-PBS

Synonyme

250708A2

---

• 

  IF Staining of Vero using 85780-2-RR (same clone as 85780-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Live vaccines of TGEV and PEDV-infected Vero cells using dsRNA antibody (85780-2-RR, Clone: 250708A2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85780-2-PBS in a different storage buffer formulation.

• 

  IF Staining of Transfected Vero using 85780-2-RR (same clone as 85780-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Transfected Vero using dsRNA antibody (85780-2-RR, Clone: 250708A2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85780-2-PBS in a different storage buffer formulation.

• 

  FC experiment of Transfected Vero using 85780-2-RR (same clone as 85780-2-PBS)

  1x10^6 Transfected Vero were intracellularly stained with 0.25 ug dsRNA Recombinant antibody (85780-2-RR, Clone:250708A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), and 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85780-2-PBS in a different storage buffer formulation.

• 

  Dot Blot experiment using 85780-2-RR (same clone as 85780-2-PBS)

  Dot blot analysis was used to confirm the specificity of 85780-2-RR anti-dsRNA antibody. samples were spotted onto NC and probed with antibody at 5 µg/ml.The amount of sample (μg) spotted is indicated next to each row. This data was developed using the same antibody clone with 85780-2-PBS in a different storage buffer formulation.

• 

  Dot Blot experiment of Poly(I:C) using 85780-2-RR (same clone as 85780-2-PBS)

  Dot blot analysis was used to confirm the specificity of 85780-2-RR dsRNA antibody. samples were spotted onto NC and probed with antibody at 1 µg/ml.The amount of sample (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 85780-2-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

85780-2-PBS bindet in IF/ICC, FC (Intra), Dot Blot, Indirect ELISA dsRNA und zeigt Reaktivität mit n/a

• Getestete Reaktivität: n/a
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: dsRNA
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

dsRNA is formed by the base pairing of two RNA strands, creating a double helix structure similar to DNA, but with some differences. dsRNA can be generated through various mechanisms. In the context of viral infections, it can be derived from the genomes of dsRNA viruses, replication intermediates of single-stranded RNA viruses, or bidirectional transcription of certain DNA viruses. Additionally, aberrant splicing or transcriptional processes may lead to the formation of dsRNA structures. dsRNA is recognized as a pathogen-associated molecular pattern (PAMP) by the immune system. It can trigger a series of immune responses in cells, including the activation of antiviral innate immune pathways.