Dynamitin (69-376 AA) Polyklonaler Antikörper

Dynamitin (69-376 AA) Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10030-2-AP

Synonyme

DCTN 50, DCTN2, DCTN50, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, DYNAMITIN, Dynamitin (69-376 AA), p50 dynamitin, RBP50

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 10030-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10030-2-AP (Dynamitin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10030-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10030-2-AP (Dynamitin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10030-2-AP

  IP Result of anti-Dynamitin (IP:10030-2-AP, 4ug; Detection:10030-2-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 10030-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10030-2-AP (Dynamitin (69-376 AA) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10030-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10030-2-AP (Dynamitin (69-376 AA) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 10030-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10030-2-AP (Dynamitin (69-376 AA) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 10030-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10030-2-AP (Dynamitin (69-376 AA) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

10030-2-AP bindet in WB, IP, IHC, ELISA Dynamitin (69-376 AA) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: dynactin 2 (p50)
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC000718
• Gene symbol: DCTN2
• Gene ID (NCBI): 10540
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is against the internal region (69-376aa) of full length p50 dynamitin.