GFP tag Polyklonaler Antikörper

GFP tag Polyklonal Antikörper für WB, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

aequorea victoria, rekombinanten Protein und mehr (6)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, RIP, IP-MS, ELISA

Konjugation

Unkonjugiert

Publikationen(848)

Rezensionen (16)

Kat-Nr. : 50430-2-AP

Synonyme

eGFP, GFP, CFP, eYFP, GFP-tag

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  WB analysis of recombinant protein using 50430-2-AP

  Western blot of eGFP-tagged fusion protein with anti-eGFP-tag (50430-2-AP) at various dilutions.

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  WB analysis of Transfected HEK-293 using 50430-2-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 50430-2-AP (GFP tag Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Transfected HEK-293T using 50430-2-AP

  IP result of anti-GFP tag (IP:50430-2-AP, 4ug; Detection:50430-2-AP 1:10000) with Transfected HEK-293T cells lysate 400 ug.

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  IF Staining of mouse brain using 50430-2-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded transgenic mouse brain tissue using GFP tag antibody (50430-2-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Transfected HEK-293 using 50430-2-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed Transfected HEK-293 cells using 50430-2-AP (GFP tag antibody) at dilution of 1:100 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of Transfected HEK-293 using 50430-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using GFP tag antibody (50430-2-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: recombinant protein, Transfizierte HEK-293-Zellen
• Erfolgreiche IP: Transfected HEK-293T cells
• Erfolgreiche Detektion in IF-P: transgenic mouse brain tissue
• Erfolgreiche Detektion in IF/ICC: Transfizierte HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 489 publications below
• IHC: See 14 publications below
• IF: See 163 publications below
• IP: See 91 publications below
• CoIP: See 64 publications below
• ChIP: See 8 publications below
• RIP: See 3 publications below

Produktinformation

50430-2-AP bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, RIP, IP-MS, ELISA GFP tag und zeigt Reaktivität mit aequorea victoria, rekombinanten Protein

• Getestete Reaktivität: aequorea victoria, rekombinanten Protein
• In Publikationen genannte Reaktivität: Hausschwein, Hefe, Hund, Maus, Ratte, Seidenraupe
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GFP tag fusion protein Ag2128
• Vollständiger Name: GFP tag
• Berechnetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: M62653
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a rabbit polyclonal antibody, generated against the full-length eGFP protein. It exhibits reactivity towards variants of Aequorea victoria GFP, including S65T-GFP, RS-GFP, YFP, CFP, and eGFP.

Publikationen

Species	Application	Title
	WB	Nat Biotechnol; Programmable protein expression using a genetically encoded m6A sensor; Authors - Bahjat F Marayati
	WB	Signal Transduct Target Ther; Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis; Authors - Chuan Yang
	WB	Cell; Phase separation drives RNA virus-induced activation of the NLRP6 inflammasome.; Authors - Chen Shen
	WB,IF	Gastroenterology; PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis; Authors - Tian-Yi Jiang
	WB	Nat Genet; Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome.; Authors - Margot A Cousin
	WB,CoIP	Mol Plant; A TT1-SCE1 module integrates ubiquitination and SUMOylation to regulate heat tolerance in rice; Authors - Hong-Xiao Yu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Manon (Verified Customer) (09-25-2025)

It works well

• Applications: Western Blot
• Cell Tissue Type: Fibroblasts

FH

Ioana (Verified Customer) (08-27-2025)

I have used this antibody for a lot of different applications and it has performed well throughout. I was particularly impressed with it's efficiency in doing IP experiments.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: HeLaM, NRK

FH

Yi (Verified Customer) (07-23-2025)

This GFP antibody performs reliably in both Western blot and co-IP applications. It shows strong specificity and sensitivity for GFP-tagged proteins, with clean results and minimal background for detecting and pulling down GFP fusion proteins.

• Applications: Immunoprecipitation
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: human

FH

Kamal (Verified Customer) (02-27-2025)

Liver lysates were subjected to SDS-PAGE and immunoblotted with GFP antibody. Multiple non-specific protein bands were detected in liver tissue lysates.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Liver tissue

GFP tag Antibody Western Blot validation (1:2000 dilution) in Liver tissue (Cat no:50430-2-AP)

FH

Raquel (Verified Customer) (07-12-2024)

Immunofluorescence analysis in cryostat sections of 4% PFA fixed human brain organoid derived from iPSCs expressing YFP with 50430-2-AP GFP tag antibody at dilution of 1:100 (under 20x lens). (Green:YFP; Magenta: GFP tag antibody)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human brain organoids

GFP tag Antibody Immunofluorescence validation (1:100 dilution) in Human brain organoids (Cat no:50430-2-AP)

FH

S (Verified Customer) (05-26-2023)

Excellent

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 500
• Cell Tissue Type: AC16

GFP tag Antibody Western Blot,Immunofluorescence validation (500 dilution) in AC16 (Cat no:50430-2-AP)

FH

PK (Verified Customer) (03-20-2023)

Very Good

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: HEK

GFP tag Antibody Western Blot validation (1000 dilution) in HEK (Cat no:50430-2-AP)

FH

Stephen (Verified Customer) (08-02-2022)

HeLa cells transfected with plasmid expressing EGFP fixed with 4% PFA for 15 mins and immunostained with rabbit GFP antibody at 4 degrees incubated for 20h washed then incubated secondary alexa 594 and DAPI for 1 hour room temp imaged on wide field fluorescence microscope.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

GFP tag Antibody Immunofluorescence validation (1:1000 dilution) in HeLa (Cat no:50430-2-AP)

FH

A (Verified Customer) (01-04-2022)

Works for IP

• Applications: Immunoprecipitation
• Cell Tissue Type: C. elegans

FH

Rinalda (Verified Customer) (06-21-2021)

Great product

• Applications: Western Blot
• Cell Tissue Type: fungal cells

FH

Lana (Verified Customer) (12-22-2020)

SDS-PAGE: 15 ug/ul RIPA protein lysate, 4-12% Bis-Tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) for 2h at 80V, 4C.Blocking: SEA Block Blocking Buffer 1h, room T.Primary Ab: O/N incubation at 4C, 1:2500.Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.Lines of WB image: 1 – protein ladder, 2 – HEK293 whole cell lysate, negative transfection, 3 – whole cell lysate of cells transfected with eGFP.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: HEK293

GFP tag Antibody Western Blot validation (1:2500 dilution) in HEK293 (Cat no:50430-2-AP)

FH

Paul (Verified Customer) (01-15-2020)

Works well for WB.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FH

Laura (Verified Customer) (01-14-2020)

Good sensitivity for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FH

Aamir (Verified Customer) (01-08-2020)

Works well for WB and IF

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1000
• Cell Tissue Type: HEKs

FH

Erica (Verified Customer) (09-26-2019)

This antibody works very well with both western blots and IP. We previously used GFP antibody from another company and it didn't work well. We then switched to Proteinntech's and the signal was very strong! Highly recommend!

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:2000 WB
• Cell Tissue Type: HEK, MEF, HELA

FH

Mayur (Verified Customer) (04-30-2019)

The H4 cells were transfected with pcDNA only and pcDNA EGFP . The expression of the EGFP was measured using the GFP antibody from ProteinTech. Great antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5K
• Cell Tissue Type: H4 cells

GFP tag Antibody Western Blot, validation (1:5K dilution) in H4 cells (Cat no:50430-2-AP)