GFP tag Monoklonaler Antikörper

GFP tag Monoklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

rekombinanten Protein und mehr (4)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(605)

CloneNo.

1E10H7

Rezensionen (22)

Kat-Nr. : 66002-1-Ig

Synonyme

eGFP, GFP, CFP, eYFP, GFP-tag

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  WB analysis using 66002-1-Ig

  HEK293 cells, CHO cells, normal mouse brain and GFP transgenic mouse brain were subjected to SDS PAGE followed by western blot with 66002-1-Ig (GFP tag antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of recombinant protein using 66002-1-Ig

  WB result of eGFP fusion protein with anti-eGFP tag (66002-1-Ig) with GFP fusion protein at varous dilutions.

• 

  WB analysis of Recombinant protein using 66002-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 66002-1-Ig (GFP tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 66002-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 66002-1-Ig (GFP tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Transfected HEK-293T using 66002-1-Ig

  IP result of anti-GFP tag (IP:66002-1-Ig, 4ug; Detection:66002-1-Ig 1:10000) with Transfected HEK-293T cells lysate 400 ug.

• 

  IF Staining of Transfected HEK-293 using 66002-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using GFP tag antibody (66002-1-Ig, Clone: 1E10H7 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: GFP transgenic mouse brain tissue, Recombinant protein
• Erfolgreiche IP: Transfected HEK-293T cells
• Erfolgreiche Detektion in IF/ICC: Transfizierte HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 396 publications below
• IHC: See 8 publications below
• IF: See 75 publications below
• IP: See 63 publications below
• ELISA: See 1 publications below
• CoIP: See 31 publications below
• ChIP: See 5 publications below
• RIP: See 2 publications below

Produktinformation

66002-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA GFP tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GFP tag fusion protein Ag2128
• Vollständiger Name: GFP tag
• Berechnetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: M62653
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP.

Publikationen

Species	Application	Title
	WB,IP	Cell; The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins; Authors - Huaqing Deng
		Nature; The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis.; Authors - Kirill Bersuker
	WB,IF	Cell Discov; Glc7/PP1 dephosphorylates histone H3T11 to regulate autophagy and telomere silencing in response to nutrient availability; Authors - Xinyu Zhang
		Cell Host Microbe; Microbiota-derived urocanic acid triggered bytyrosine kinase inhibitors potentiates cancer immunotherapy efficacy; Authors - Mengying Zhang
	WB,CoIP	Cell Metab; Protein O-GlcNAcylation and hexokinase mitochondrial dissociation drive heart failure with preserved ejection fraction; Authors - Yuki Tatekoshi
	WB	Nat Genet; Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome.; Authors - Margot A Cousin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Javier (Verified Customer) (09-09-2025)

Perfect antibody for Wb and IP assays.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mice kidneys

FH

Ioana (Verified Customer) (08-27-2025)

This antibody works well in Western Blotting and amplifying signal after PFA fixation during IF.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HeLaM, NRK

FH

Anastasia (Verified Customer) (08-07-2024)

Zebrafish embryos expressing cytosolic GFP

• Applications: Western Blot
• Cell Tissue Type: Zebrafish embryos

GFP tag Antibody Western Blot validation ( dilution) in Zebrafish embryos (Cat no:66002-1-Ig)

FH

Amy (Verified Customer) (02-11-2023)

Detected EGFP-tagged constructs expressed in HEK293T cells at similar strength to other antibodies however with slightly more background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:20,000
• Cell Tissue Type: HEK293T

GFP tag Antibody Western Blot validation (1:20,000 dilution) in HEK293T (Cat no:66002-1-Ig)

FH

Tom (Verified Customer) (02-03-2023)

Works great. No problem. Love proteintech.

• Applications: Western Blot
• Primary Antibody Dilution: 1:25000
• Cell Tissue Type: Bacteria

FH

Tatyana (Verified Customer) (01-09-2023)

Good signal, antibody can be reused. Overexpressed GFP-tagged proteins (and GFP alone) were pulled down using GFP-Trap beads and WB was done on lysates and IP samples. Antibody was diluted in 4% milk (and could be reused several times).

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human / HeLa cells

GFP tag Antibody Western Blot,Immunoprecipitation validation (1:2000 dilution) in Human / HeLa cells (Cat no:66002-1-Ig)

FH

Sheila (Verified Customer) (07-22-2022)

It works very well

• Applications: Western Blot, Immunoprecipitation
• Cell Tissue Type: Murine Breast cancer

FH

Juliana (Verified Customer) (01-28-2022)

works great for Western blot!

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: HEK293T

FH

Prasanna (Verified Customer) (01-04-2021)

Of the 3 antibodies tested for GFP this one gave the cleanest and strongest signal by western blot. When I saw it on sale, I bought 8!

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: Hap1, N2a (mouse neuroblastoma)

FH

Lana (Verified Customer) (12-22-2020)

SDS-PAGE: 15 ug/ul RIPA protein lysate, 4-12% Bis-Tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) for 2h at 80V, 4C.Blocking: SEA Block Blocking Buffer 1h, room T.Primary Ab: O/N incubation at 4C, 1:5000.Secondary Ab: IRDye 800CW Goat anti-Mouse, 1:15000.Lines of WB image: 1 – protein ladder, 2 – HEK293 whole cell lysate, negative transfection, 3 – whole cell lysate of cells transfected with eGFP.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293

GFP tag Antibody Western Blot validation (1:5000 dilution) in HEK293 (Cat no:66002-1-Ig)

FH

Thomas (Verified Customer) (11-19-2020)

HEK293T and HEK293T stably transfected with pAcGFP plasmid. 10ug total protein loaded per well. Membrane blocked 1 hour in 5% BSA prior to anti-GFP (1:2000) o/n at 4 degrees. Goat anti-mouse HRP secondary (1:10,000) used.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

GFP tag Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:66002-1-Ig)

FH

Jane (Verified Customer) (03-02-2020)

Adenovirus-GFP infected cardiomyocytes stained with GFP antibody, signal is strong and effective

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: cardiomyocyte culture

FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

• Applications: Immunofluorescence,
• Cell Tissue Type: HEART

FH

Jie (Verified Customer) (01-27-2020)

Worked for western blot with GFP-LC3 transfected cardiomyocytes

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cardiomyocytes

FH

Paul (Verified Customer) (01-15-2020)

Works well for Westerns.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: HEK293T

FH

Laura (Verified Customer) (01-15-2020)

Good antibody for Western Blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293T

FH

Aamir (Verified Customer) (01-08-2020)

Worked well for WB

• Applications: Western Blot,
• Primary Antibody Dilution: 5000
• Cell Tissue Type: HEKs

FH

Benjamin (Verified Customer) (01-07-2020)

Easily detects recombinant GFP protein via western blot with very little background.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2500 to 1:5000
• Cell Tissue Type: Recombinant GFP protein

FH

Jing (Verified Customer) (01-03-2020)

Used this to detect tranduce efficiency of the AAV-GFP virus. with 5% non-fat milk, there are two bands around 20-30kd, not sure which one is correct. And the antibody is relative weak, has to use 1:500 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse heart

FH

Shan (Verified Customer) (12-25-2019)

The GFP antibody showed great sensitivity for WB and it was easily detecable. But it was insteresting that when the SDS PAGE gel separating gel concentration reeached to 12%, you can see two bands at ~30kDa and ~20kDa.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: 293T

GFP tag Antibody Western Blot, validation (1:2500 dilution) in 293T (Cat no:66002-1-Ig)

FH

Jason (Verified Customer) (11-04-2019)

This is a good antibody for detecting GFP tag on Western blot, using 1:1000 dilution. The price is unbeatable, worth each penny.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: GFP-tagged protein expressed in human cell lines

FH

Hend (Verified Customer) (10-14-2019)

antibody used in western blot against egfp labelled protien and clear band was detected.

• Applications: Western Blot,
• Primary Antibody Dilution: 1 to 4000