Collagen Type XVIII Polyklonaler Antikörper

Collagen Type XVIII Polyklonal Antikörper für WB, IHC, IF, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Zebrafisch und mehr (1)

Anwendung

WB, IHC, IF, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Rezensionen (1)

Kat-Nr. : 18301-1-AP

Synonyme

COL18, COL18A1, Endostatin, Collagen alpha 1(XVIII) chain, Collagen alpha-1(XVIII) chain

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  WB analysis of human kidney using 18301-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 18301-1-AP (Endostatin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 18301-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18301-1-AP (Endostatin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 18301-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18301-1-AP (Endostatin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse eye using 18301-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Collagen Type XVIII antibody (18301-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of zebrafish retina using 18301-1-AP

  Immunofluorescence analysis of Collagen18a1 (aka Endostatin) polyclonal antibody (18301-1-AP, green) staining on tissue sections of the zebrafish retina at 3 days post fertilisation. Dilution 1:200. Tissue fixed overnight in 4% PFA. DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK.

• 

  IF Staining of zebrafish retina using 18301-1-AP

  Immunofluorescence analysis of the zebrafish retina at 5 days post fertilisation. Collagen18a1 (aka Endostatin) polyclonal antibody (18301-1-AP, magenta). Dilution 1:200. Glutamine synthetase (66323-1-Ig, green) dilution 1:500. Tissue fixed overnight in 4% PFA; DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Augengewebe
• Erfolgreiche Detektion in IF: zebrafish retina

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

18301-1-AP bindet in WB, IHC, IF, IF-P, ELISA Collagen Type XVIII und zeigt Reaktivität mit human, Maus, Zebrafisch

• Getestete Reaktivität: human, Maus, Zebrafisch
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: collagen, type XVIII, alpha 1
• Berechnetes Molekulargewicht: 178 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC063833
• Gene symbol: COL18A1/Endostatin
• Gene ID (NCBI): 80781
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COL18A1 is a naturally-occurring 20-kDa C-terminal fragment derived from collagen alpha-1(XVIII) chain. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. It potently inhibits endothelial cell proliferation and inhibits endothelial cell angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. COL18A1 is a broad spectrum angiogenesis inhibitor and may interfere with the pro-angiogenic action of growth factors such as basic fibroblast growth factor (bFGF/FGF-2) and vascular endothelial growth factor (VEGF). This antibody raised against 1693-1706 aa of human collagen alpha-1(XVIII) chain can recognize endostatin (20 kDa) and several endostatin-related fragments of higher molecular weight (PMID: 15545970; 19358707).

Publikationen

Species	Application	Title
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	WB	Cancer Lett; Systemic administration of mesenchymal stem cells loaded with a novel oncolytic adenovirus carrying IL-24/endostatin enhances glioma therapy.; Authors - Junhe Zhang
human	WB	FASEB J; MicroRNA-3064-5p sponged by MALAT1 suppresses angiogenesis in human hepatocellular carcinoma by targeting the FOXA1/CD24/Src pathway.; Authors - Pei Zhang
human	WB	Biotechnol Lett; PMID: 26830094; Authors - Baolong Wang
rat	WB	Neural Regen Res; miR-181b promotes angiogenesis and neurological function recovery after ischemic stroke; Authors - Li-Xia Xue

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aditya (Verified Customer) (06-16-2025)

cut between the region speicified by proteintech in a western blot and did not see any banding for hek lysate, may have run over or under cut region

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000