fetuin-B Polyklonaler Antikörper

fetuin-B Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 18052-1-AP

Synonyme

FETUB, 16G2, Gugu, Fetuin-like protein IRL685, FetuinB

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  WB analysis of human plasma using 18052-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 18052-1-AP (fetuin-B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 18052-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 18052-1-AP (fetuin-B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 18052-1-AP

  IP result of anti-fetuin-B (IP:18052-1-AP, 4ug; Detection:18052-1-AP 1:2000) with human plasma lysate 4000 ug.

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  IHC staining of human liver cancer using 18052-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18052-1-AP (fetuin-B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18052-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18052-1-AP (fetuin-B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 18052-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 18052-1-AP (fetuin-B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse liver using 18052-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 18052-1-AP (fetuin-B antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Blutgewebe, human plasma
• Erfolgreiche IP: humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• ELISA: See 1 publications below

Produktinformation

18052-1-AP bindet in WB, IHC, IP, ELISA fetuin-B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: fetuin-B fusion protein Ag12562
• Vollständiger Name: fetuin B
• Berechnetes Molekulargewicht: 382 aa, 42 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC074734
• Gene symbol: Fetuin B
• Gene ID (NCBI): 26998
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fetuins A and B are both members of the cystatin superfamily. They are mainly expressed in the liver (hepatokines), as well as other tissues such as the kidneys, tongue, and placenta. Fetuin-B has been identified as a novel adipokine/hepatokine that significantly increases hepatic steatosis and mediates impaired insulin action and glucose intolerance. (PMID: 36915725, PMID: 35702137)

Publikationen

Species	Application	Title
human	ELISA	Diabetes Metab Syndr Obes; Plasma Fetuin-B Levels are Associated with Nervous Symptoms and Conduction Velocity in Patients with Painful DPN; Authors - Ke Lin