fetuin-B Monoklonaler Antikörper

fetuin-B Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Kaninchen und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

2H7D11

Kat-Nr. : 67002-1-Ig

Synonyme

FETUB, Fetuin-like protein IRL685, FetuinB, fetuin B, 2H7D11

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  WB analysis of human blood using 67002-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 67002-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit serum using 67002-1-Ig

  rabbit serum were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 67002-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67002-1-Ig (fetuin-B antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 67002-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67002-1-Ig (fetuin-B antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 67002-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using fetuin-B antibody (67002-1-Ig, Clone: 2H7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 67002-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using fetuin-B antibody (67002-1-Ig, Clone: 2H7D11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human plasma, human blood, rabbit serum
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Leberkarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

67002-1-Ig bindet in WB, IHC, IF/ICC, IF-P, ELISA fetuin-B und zeigt Reaktivität mit human, Kaninchen

• Getestete Reaktivität: human, Kaninchen
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: fetuin-B fusion protein Ag12463
• Vollständiger Name: fetuin B
• Berechnetes Molekulargewicht: 382 aa, 42 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC074734
• Gene symbol: Fetuin B
• Gene ID (NCBI): 26998
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fetuins A and B are both members of the cystatin superfamily. They are mainly expressed in the liver (hepatokines), as well as other tissues such as the kidneys, tongue, and placenta. Fetuin-B has been identified as a novel adipokine/hepatokine that significantly increases hepatic steatosis and mediates impaired insulin action and glucose intolerance. (PMID: 36915725, PMID: 35702137)

Publikationen

Species	Application	Title
human,mouse	WB	J Agric Food Chem; Baicalein Enhances the Effect of Acarbose on the Improvement of Nonalcoholic Fatty Liver Disease Associated with Prediabetes via the Inhibition of De Novo Lipogenesis.; Authors - Yan Xing
mouse	WB	J Proteome Res; Novel Regulators of Retina Neovascularization: A Proteomics Approach.; Authors - Manhong Xu
mouse	WB	Front Pharmacol; Delineation of renal protein profiles in aristolochic acid I-induced nephrotoxicity in mice by label-free quantitative proteomics; Authors - Xinhui Liu
human	IHC	Mol Cell Proteomics; Proteomic analysis of human follicular fluid-derived exosomes reveals that insufficient folliculogenesis in aging women is associated with infertility; Authors - Zhen Liu