fetuin-B Monoklonaler Antikörper

fetuin-B Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Kaninchen

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H7D11

Kat-Nr. : 67002-1-PBS

Synonyme

FETUB, Fetuin-like protein IRL685, FetuinB, fetuin B, 2H7D11

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  WB analysis of human blood using 67002-1-Ig (same clone as 67002-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 67002-1-Ig (same clone as 67002-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit serum using 67002-1-Ig (same clone as 67002-1-PBS)

  rabbit serum were subjected to SDS PAGE followed by western blot with 67002-1-Ig (fetuin-B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67002-1-Ig (same clone as 67002-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67002-1-Ig (fetuin-B antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67002-1-Ig (same clone as 67002-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67002-1-Ig (fetuin-B antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67002-1-Ig (same clone as 67002-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using fetuin-B antibody (67002-1-Ig, Clone: 2H7D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67002-1-Ig (same clone as 67002-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using fetuin-B antibody (67002-1-Ig, Clone: 2H7D11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67002-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67002-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA fetuin-B und zeigt Reaktivität mit human, Kaninchen

• Getestete Reaktivität: human, Kaninchen
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: fetuin-B fusion protein Ag12463
• Vollständiger Name: fetuin B
• Berechnetes Molekulargewicht: 382 aa, 42 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC074734
• Gene symbol: Fetuin B
• Gene ID (NCBI): 26998
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fetuins A and B are both members of the cystatin superfamily. They are mainly expressed in the liver (hepatokines), as well as other tissues such as the kidneys, tongue, and placenta. Fetuin-B has been identified as a novel adipokine/hepatokine that significantly increases hepatic steatosis and mediates impaired insulin action and glucose intolerance. (PMID: 36915725, PMID: 35702137)