Gamma Catenin Polyklonaler Antikörper

Gamma Catenin Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 27872-1-AP

Synonyme

ARVD12, Catenin gamma, CTNNG, Desmoplakin 3, Desmoplakin III, DP3, DPIII, junction plakoglobin, JUP, PDGB, PKGB, γ-Catenin

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Galerie der Validierungsdaten

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  WB analysis using 27872-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27872-1-AP (Gamma Catenin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27872-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27872-1-AP (gamma catenin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 27872-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 27872-1-AP (gamma catenin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27872-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27872-1-AP (gamma catenin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27872-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27872-1-AP (Gamma Catenin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27872-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27872-1-AP (Gamma Catenin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 27872-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 27872-1-AP (gamma catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 27872-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Gamma Catenin antibody (27872-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 27872-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Gamma Catenin antibody (27872-1-AP) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of MCF-7 using 27872-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 27872-1-AP (gamma catenin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, HUVEC-Zellen, Mausherzgewebe, MCF-7-Zellen, Rattenherzgewebe, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen, A431-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

27872-1-AP bindet in WB, IHC, IF, ELISA Gamma Catenin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Gamma Catenin fusion protein Ag27394
• Vollständiger Name: junction plakoglobin
• Beobachtetes Molekulargewicht: 82 kDa
• GenBank-Zugangsnummer: BC000441
• Gene symbol: JUP
• Gene ID (NCBI): 3728
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Oncol (Dordr); The POU2F1/miR-4490/USP22 axis regulates cell proliferation and metastasis in gastric cancer.; Authors - Yizhi Xiao
human	WB,IF	Int J Biol Macromol; Frameshift variants in the C-terminal of CTNNB1 cause familial exudative vitreoretinopathy by AXIN1-mediated ubiquitin-proteasome degradation condensation; Authors - Yining Liu