gephyrin Polyklonaler Antikörper

gephyrin Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12681-1-PBS

Synonyme

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  WB analysis using 12681-1-AP (same clone as 12681-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12681-1-AP (gephyrin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 12681-1-AP (same clone as 12681-1-PBS)

  IP result of anti-gephyrin (IP:12681-1-AP, 4ug; Detection:12681-1-AP 1:700) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 12681-1-AP (same clone as 12681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12681-1-AP (gephyrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 12681-1-AP (same clone as 12681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12681-1-AP (gephyrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 12681-1-AP (same clone as 12681-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 12681-1-AP (gephyrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12681-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12681-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA gephyrin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: gephyrin fusion protein Ag3370
• Vollständiger Name: gephyrin
• Berechnetes Molekulargewicht: 769 aa, 83 kDa
• Beobachtetes Molekulargewicht: 93 kDa
• GenBank-Zugangsnummer: BC030016
• Gene symbol: GPHN
• Gene ID (NCBI): 10243
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Gephyrin (GPHN) is an organizational protein that clusters and localizes the inhibitory glycine receptor (GlyR) and GABAA receptors to the microtubular matrix of the neuronal postsynaptic membrane. Mice deficient in gephyrin develop a hereditary molybdenum cofactor deficiency and a neurological phenotype that mimics startle disease (hyperekplexia). In non-neuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Two isoforms produced by alternative splicing have been described. The observed MW of Gephyrin is 93 kDa, larger than the predicated of 83 kDa, which may be due to the modifications on various phosphorylation sites.