gp130/IL6ST Rekombinanter Antikörper

gp130/IL6ST Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241340A11

Kat-Nr. : 84113-5-PBS

Synonyme

IL6RB, IL-6R subunit beta, IL-6 receptor subunit beta, gp130, CD130

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  WB analysis using 84113-5-RR (same clone as 84113-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84113-5-RR (gp130/IL6ST antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84113-5-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01049-4

  Sandwich ELISA standard curve of MP01049-4, Rat gp130/IL6ST Recombinant Matched Antibody Pair - PBS only. 84113-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0857. 84113-5-PBS was HRP conjugated as the detection antibody. Range: 78.1-2500 pg/mL

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  Sample test of MP01049-4

  The mean gp130/IL6ST concentration was determined to be 24.02 ng/mL in C6 cell extract based on a 2.0 mg/mL extract load, 27.20 ng/mL in PC-12 cell extract based on a 1.4 mg/mL extract load and 16.64 ng/mL in rat brain tissue extract based on a 1.6 mg/mL extract load.

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  Affinity and Kinetic Characterization of 84113-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84113-5-RR against Rat gp130/IL6ST were performed. The affinity constant is 1.04 nM.

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Geprüfte Anwendungen

Produktinformation

84113-5-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test gp130/IL6ST und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: interleukin 6 signal transducer
• Berechnetes Molekulargewicht: 102 kDa
• Beobachtetes Molekulargewicht: 90-150 kDa
• GenBank-Zugangsnummer: NM_001008725.3
• Gene symbol: Il6st
• Gene ID (NCBI): 25205
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). The binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells (PMID: 9815830).