gp130/IL6ST Rekombinanter Antikörper

gp130/IL6ST Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242236E1

Kat-Nr. : 84794-4-RR

Synonyme

242236E1, CD130, gp130, IL-6 receptor subunit beta, IL-6R subunit beta

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  WB analysis using 84794-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84794-4-RR (gp130/IL6ST antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84794-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84794-4-RR against Mouse gp130/IL6ST were performed. The affinity constant is 89.7 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, C2C12-Zellen, Neuro-2a-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84794-4-RR bindet in WB, ELISA gp130/IL6ST und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: gp130/IL6ST fusion protein Eg1631
• Vollständiger Name: interleukin 6 signal transducer
• Berechnetes Molekulargewicht: 102kDa, 917aa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: NM_010560.3
• Gene symbol: Il6st
• Gene ID (NCBI): 16195
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GP130, also known as IL6ST, is a transmembrane protein that serves as a shared signal-transducing subunit for a variety of cytokines(PMID: 39414364), including interleukin-6 (IL-6), IL-11, IL-27, leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), oncostatin M (OSM), cardiotrophin-1 (CT-1), cardiotrophin-like cytokine (CLC)/CLC factor 1 (CLCF-1), and neuropoietin (PMID: 24099136). This protein is highly conserved among vertebrates and plays a crucial role in mediating interactions between a wide range of immune and non-immune cells. GP130 was originally discovered in 1978 and was successfully cloned and expressed in 1990, leading to a deeper understanding of cytokine signaling pathways (PMID: 308233).