gp130/IL6ST Rekombinanter Antikörper

gp130/IL6ST Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242236B6

Kat-Nr. : 98275-1-PBS

Synonyme

242236B6, CD130, gp130, IL-6 receptor subunit beta, IL-6R subunit beta

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  FC experiment of C57BL/6 mouse splenocytes using 98275-1-RR (same clone as 98275-1-PBS)

  1x10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse gp130/IL6ST Rabbit Recombinant Antibody (98275-1-RR, Clone: 242236B6) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD3. Cells were not fixed. This data was developed using the same antibody clone with 98275-1-PBS in a different storage buffer formulation.

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  FC experiment of C57BL/6 mouse splenocytes using 98275-1-RR (same clone as 98275-1-PBS)

  1x10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse gp130/IL6ST Rabbit Recombinant Antibody (98275-1-RR, Clone: 242236B6) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98275-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98275-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98275-1-RR against Mouse gp130/IL6ST were performed. The affinity constant is 3.60 nM.

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Geprüfte Anwendungen

Produktinformation

98275-1-PBS bindet in FC gp130/IL6ST und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: gp130/IL6ST fusion protein Eg1631
• Vollständiger Name: interleukin 6 signal transducer
• Berechnetes Molekulargewicht: 102kDa, 917aa
• GenBank-Zugangsnummer: NM_010560.3
• Gene symbol: Il6st
• Gene ID (NCBI): 16195
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). The binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells (PMID: 9815830).