gp130/IL6ST Rekombinanter Antikörper

gp130/IL6ST Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242236B6

Kat-Nr. : 98275-1-RR

Synonyme

242236B6, CD130, gp130, IL-6 receptor subunit beta, IL-6R subunit beta

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  FC experiment of C57BL/6 mouse splenocytes using 98275-1-RR

  1x10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse gp130/IL6ST Rabbit Recombinant Antibody (98275-1-RR, Clone: 242236B6) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD3. Cells were not fixed.

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  FC experiment of C57BL/6 mouse splenocytes using 98275-1-RR

  1x10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse gp130/IL6ST Rabbit Recombinant Antibody (98275-1-RR, Clone: 242236B6) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98275-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98275-1-RR against Mouse gp130/IL6ST were performed. The affinity constant is 3.60 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: C57BL/6 mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98275-1-RR bindet in FC gp130/IL6ST und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: gp130/IL6ST fusion protein Eg1631
• Vollständiger Name: interleukin 6 signal transducer
• Berechnetes Molekulargewicht: 102kDa, 917aa
• GenBank-Zugangsnummer: NM_010560.3
• Gene symbol: Il6st
• Gene ID (NCBI): 16195
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). The binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells (PMID: 9815830).