GST Tag Polyklonaler Antikörper

GST Tag Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein und mehr (2)

Anwendung

WB, IP, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

188

Rezensionen (5)

Kat-Nr. : 10000-0-AP

Synonyme

Glutathione S-Transferase, GST, gst tag

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• Galerie der Validierungsdaten
• Proteintech vs. Other Vendors

• 

  WB analysis of recombinant protein using 10000-0-AP

  Western blot of GST-tagged fusion protein with anti-GST-tag (10000-0-AP) at various dilutions.

• 

  FC experiment of HeLa using 10000-0-AP

  1X10^6 HeLa cells were stained with 0.20 ug PSMD9 antibody (26922-1-AP, red) and 0.20 ug Rabbit IgG control (10000-0-AP, blue). Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 125 publications below
• IF: See 2 publications below
• IP: See 18 publications below
• ELISA: See 1 publications below
• CoIP: See 4 publications below
• ChIP: See 2 publications below

Produktinformation

10000-0-AP bindet in WB, IP, IF, CoIP, ChIP, ELISA GST Tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: GST Tag
• Berechnetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: FN315687
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.

Publikationen

Species	Application	Title
human	WB,IP	Signal Transduct Target Ther; SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis; Authors - Saisai Guo
human	WB	Gastroenterology; PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis; Authors - Tian-Yi Jiang
		Ann Rheum Dis; Autoantibodies identify primary Sjögren's syndrome in patients lacking serum IgG specific for Ro/SS-A and La/SS-B; Authors - Sherri Longobardi
	WB	Immunity; Targeting 7-Dehydrocholesterol Reductase Integrates Cholesterol Metabolism and IRF3 Activation to Eliminate Infection.; Authors - Jun Xiao
	WB,IP	Nat Struct Mol Biol; CRISPR-Cas9-based functional interrogation of unconventional translatome reveals human cancer dependency on cryptic non-canonical open reading frames; Authors - Caishang Zheng
human	WB	Nat Commun; PHGDH arginine methylation by PRMT1 promotes serine synthesis and represents a therapeutic vulnerability in hepatocellular carcinoma; Authors - Kui Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniel (Verified Customer) (06-10-2022)

We use this anti-GST antibody for GST fusion protein-lipid interactions. We found this antibody gives much less background than other antibodies.

• Applications: Other
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: not applicable

GST Tag Antibody Other validation (1:1000 dilution) in not applicable (Cat no:10000-0-AP)

FH

Lana (Verified Customer) (04-06-2022)

SDS-PAGE: 4-12% Bis-Tris gradient gel. Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C. Blocking: SEA Block Blocking Buffer 1h, room T. Primary Ab: O/N incubation at 4C, 1:1000. Secondary Ab: IRDye 680LT Goat anti-Rabbit, 1:15000. Lines of WB image: 1 – protein ladder, 2 – mitochondria fraction lysate, 3 – GST protein, 4 – GST fusion protein

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Lysate of Sepharose beads after GST-pull down

GST Tag Antibody Western Blot validation (1:1000 dilution) in Lysate of Sepharose beads after GST-pull down (Cat no:10000-0-AP)

FH

Shihab (Verified Customer) (10-28-2021)

Tried this antibody for a control experiment and worked nicely. Will be continuing to try this in immunofluorescence

• Applications: Western Blot
• Cell Tissue Type: Transfected HEK293

FH

Amie (Verified Customer) (07-27-2021)

I compared this antibody directly to the proteintech mouse monoclonal (66001-2-Ig). In the provided image, the first 4 lanes of each gel are identical. The rabbit antibody seems to be slightly better (less background) than the mouse.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: recombinant GST-tagged proteins expressed in yeast

GST Tag Antibody Western Blot validation (1:5000 dilution) in recombinant GST-tagged proteins expressed in yeast (Cat no:10000-0-AP)

FH

Yan (Verified Customer) (02-20-2021)

It worked very well when I performed western blot after immunoprecipitation.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse adult heart