iNOS Rekombinanter Antikörper

iNOS Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(58)

CloneNo.

6O22

Kat-Nr. : 80517-1-RR

Synonyme

NOS2, Inducible NOS, Inducible NO synthase, HEP-NOS, Hepatocyte NOS

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  WB analysis using 80517-1-RR

  Untreated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 80517-1-RR (iNOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human PBMCs using 80517-1-RR

  LPS treated human PBMCs were subjected to SDS PAGE followed by western blot with 80517-1-RR (iNOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, LPS treated human PBMCs
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Kolongewebe, humanes Lebergewebe, humanes Lungengewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 35 publications below
• IHC: See 13 publications below
• IF: See 22 publications below

Produktinformation

80517-1-RR bindet in WB, IHC, IF/ICC, ELISA iNOS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: nitric oxide synthase 2, inducible
• Berechnetes Molekulargewicht: 131 kDa
• Beobachtetes Molekulargewicht: 110-130 kDa
• GenBank-Zugangsnummer: NM_000625
• Gene symbol: iNOS
• Gene ID (NCBI): 4843
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.

Publikationen

Species	Application	Title
mouse	IF	Redox Biol; Covalent organic framework based cytoprotective therapy after ischemic stroke; Authors - Yuqin Peng
rat	WB,IF	J Control Release; Controlled extracellular vesicles release from aminoguanidine nanoparticle-loaded polylysine hydrogel for synergistic treatment of spinal cord injury; Authors - Shaoke Wang
mouse	WB,IHC,IF	J Nanobiotechnology; Lemon-derived nanoparticle-functionalized hydrogels regulate macrophage reprogramming to promote diabetic wound healing; Authors - Enyou Jin
mouse	WB	Bioeng Transl Med; Propionate-producing engineered probiotics ameliorated murine ulcerative colitis by restoring anti-inflammatory macrophage via the GPR43/HDAC1/IL-10 axis; Authors - Guangbo Kang
rat	IF	Int J Mol Sci; Ursolic Acid Alleviates Neuroinflammation after Intracerebral Hemorrhage by Mediating Microglial Pyroptosis via the NF-κB/NLRP3/GSDMD Pathway; Authors - Pan Lei
mouse	WB	Int J Mol Sci; C-X-C Motif Chemokine 3 Promotes the Inflammatory Response of Microglia after Escherichia coli-Induced Meningitis; Authors - Xinyi Qu