iNOS Rekombinanter Antikörper

iNOS Rekombinant Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

CloneNo.

6O22

Kat-Nr. : 80517-1-RR

Synonyme

HEP NOS, Hepatocyte NOS, Inducible NO synthase, Inducible NOS, INOS, NOS, NOS type II, NOS2, NOS2A

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Galerie der Validierungsdaten

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  WB analysis using 80517-1-RR

  Untreated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 80517-1-RR (iNOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human PBMCs using 80517-1-RR

  LPS treated human PBMCs were subjected to SDS PAGE followed by western blot with 80517-1-RR (iNOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 80517-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, LPS treated human PBMCs
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Kolongewebe, humanes Lebergewebe, humanes Lungengewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Produktinformation

80517-1-RR bindet in WB, IHC, IF, ELISA iNOS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: nitric oxide synthase 2, inducible
• Berechnetes Molekulargewicht: 131 kDa
• Beobachtetes Molekulargewicht: 110-130 kDa
• GenBank-Zugangsnummer: NM_000625
• Gene symbol: NOS2
• Gene ID (NCBI): 4843
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.

Publikationen

Species	Application	Title
rat	WB,IF	J Control Release; Controlled extracellular vesicles release from aminoguanidine nanoparticle-loaded polylysine hydrogel for synergistic treatment of spinal cord injury; Authors - Shaoke Wang
rat	IF	Int J Mol Sci; Ursolic Acid Alleviates Neuroinflammation after Intracerebral Hemorrhage by Mediating Microglial Pyroptosis via the NF-κB/NLRP3/GSDMD Pathway; Authors - Pan Lei
mouse	WB	Int J Mol Sci; C-X-C Motif Chemokine 3 Promotes the Inflammatory Response of Microglia after Escherichia coli-Induced Meningitis; Authors - Xinyi Qu
rat	WB	J Ethnopharmacol; Extract of Astragali Radix and Solanum nigrum Linne regulates microglia and macrophage polarization and inhibits the growth and infiltration of C6 glioblastoma; Authors - Mingxue Shen
mouse	IF,IHC	Mater Today Bio; Treatment with FAP-targeted zinc ferrite nanoparticles for rheumatoid arthritis by inducing endoplasmic reticulum stress and mitochondrial damage; Authors - Weizhong Qi
rat	IHC	Free Radic Biol Med; Exosomes derived from schwann cells alleviate mitochondrial dysfunction and necroptosis after spinal cord injury via AMPK signaling pathway-mediated mitophagy; Authors - Bo Xu