Ki-67 Polyklonaler Antikörper

Ki-67 Polyklonal Antikörper für IF/ICC, IF-P, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

IF/ICC, IF-P, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Kat-Nr. : CL647-28074

Synonyme

Mki67, ki67, Ki 67, Proliferation related Ki 67 antigen

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  IF Staining of mouse colon using CL647-28074

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 647 ki67 antibody (CL647-28074) at dilution of 1:200.

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  IF Staining of mouse colon using CL647-28074

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 647 ki67 antibody (CL647-28074) at dilution of 1:200.

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  IF Staining of HeLa using CL647-28074

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 ki67 antibody (CL647-28074) at dilution of 1:200.

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  FC experiment of Jurkat using CL647-28074

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated Ki-67 Polyclonal antibody (CL647-28074)(red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of NIH/3T3 using CL647-28074

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated Ki-67 Polyclonal antibody (CL647-28074)(red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maus-Kolongewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): NIH/3T3-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-28074 bindet in IF/ICC, IF-P, FC (Intra) Ki-67 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Ki-67 fusion protein Ag27894
• Vollständiger Name: antigen identified by monoclonal antibody Ki 67
• Berechnetes Molekulargewicht: 351 kDa
• GenBank-Zugangsnummer: NM_001081117
• Gene symbol: ki67
• Gene ID (NCBI): 17345
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.